Team:UNICAMP-Brazil/Protocols

From 2009.igem.org

(Difference between revisions)
(Protocols)
(Protocols)
 
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'''[[Team:UNICAMP-Brazil/Protocols/Electroelution|12. Electroelution]]'''
'''[[Team:UNICAMP-Brazil/Protocols/Electroelution|12. Electroelution]]'''
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'''[[Team:UNICAMP-Brazil/Protocols/Preparation of electrocompetent S. cereviseae|13. Preparation of electrocompetent S. cerevisiae]]'''
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'''[[Team:UNICAMP-Brazil/Protocols/Preparation of electrocompetent S. cereviseae|13. Preparation of electrocompetent ''S. cerevisiae'']]'''
'''[[Team:UNICAMP-Brazil/Protocols/Restriction reaction|14. Restriction Reaction]]'''
'''[[Team:UNICAMP-Brazil/Protocols/Restriction reaction|14. Restriction Reaction]]'''

Latest revision as of 18:55, 21 October 2009

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Protocols

1. Genomic DNA Extraction

2. Mini-Prep

3. Transformation

4. Preparation of electrocompetent E. coli

5. Yeast DNA Extraction

6. Yeast RNA Extraction

7. Purification of DNA fragments from agarose gels

8. Isolation of RNA using Trizol Reagent (Invitrogen)

9. SAP Dephosphorylation

10. CIAP Dephosphorylation

11. T4 DNA Ligase

12. Electroelution

13. Preparation of electrocompetent S. cerevisiae

14. Restriction Reaction

15. pGEM cloning strategy

16. Expression strategy in Yeast