Team:Wash U/Biological Parts

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Revision as of 23:06, 6 July 2009

Parts

Component	Description	Part/Accession #	Base Pairs	Plasmid	Resistance	Well
RBS	Ribosomal Binding Site	[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]	12	pSB1A2	Ampicillin	plate 1, 2M
Red Light Sensor	description	[http://partsregistry.org/Part:BBa_I15010 BBa_I15010]	2,238	pSB2K3	Kanamycin	N/A
OmpR (E. coli)	description	[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=retrieve&dopt=full_report&list_uids=947913&log$=databasead&logdbfrom=protein NP_417864.1]	720	pSB1T3	Tetracycline	N/A
Terminator	description	[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]	129	pSB1AK3	Ampicillin and Kanamycin	plate 1, 23L
OmpR + Terminator	description	sequence	916	pany-amp	Ampicillin	synthesized
OmpC promoter	description	[http://partsregistry.org/Part:BBa_R0082 BBa_R0082]	108	pSB1A2	Ampicillin	plate 1, 16K
puc B/A	description	sequence	375	pSB1A3	Ampicillin	N/A
puc B	description	[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=retrieve&dopt=full_report&list_uids=3719170&log$=databasead&logdbfrom=nuccore YP_353390]	156	?	?	N/A
puc A	description	[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=retrieve&dopt=full_report&list_uids=3719171&log$=databasead&logdbfrom=nuccore YP_353391]	165	?	?	N/A
OmpC promoter+BA	description	sequence	539	pany-kana	Kanamycin	synthesized
Light Response System	description	[http://partsregistry.org/Part:BBa_M30109 BBa_M30109]	4,333	?	Ampicillin	N/A
TetR repressible	description	[http://partsregistry.org/Part:BBa_J13002 BBa_J13002]	74	pSB1A2	Ampicillin	plate 1, 13B
Green Flourescent Protein	description	[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]	976	pSB1A2	Ampicillin	plate 1, 12M

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Characterization

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Modeling

Modeling the Gene Regulatory Network

Our group seeks to assess the optimality of the synthetic system that modulates pucBA gene expression and LH2 complex assembly in Rhodobacter sphaeroides. Here we employ a mathematical model of this system to generate predictions about the behavior of the active system in response to light input. Features of the system that the model may help investigate include the time scale of response to light signals, the robustness of the system in response to fluctuations in light intensity, and the translation between changes in gene expression and the absorbance spectrum of the engineered cells.

pucBA Expression Model Diagram

pucBA Model Reactions

References

1. Alon, Uri. Introduction to systems biology and the design principles of biological networks. Boca Raton, FL: Chapman & Hall, 2006.
2. Bower, James M. Computational Modeling of Genetic and Biochemical Networks (Computational Molecular Biology). New York: M.I.T. PRESS, 2001.
3. System modeling in cellular biology from concepts to nuts and bolts. Cambridge, MA: MIT P, 2006.
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-[[Image:pucBAModelDiagram.jpg|thumb|300px|pucBA Expression Model Diagram]]
+[[Image:pucBAModelDiagram.jpg|thumb|450px|pucBA Expression Model Diagram]]
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-[[Image:pucBAModelEq.jpg|500px]]
+[[Image:pucBAModelEq.jpg|450px]]
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-[[Image:pucBAModelRxn.jpg|thumb|200px|pucBA Model Reactions]]
+[[Image:pucBAModelRxn.jpg|thumb|450px|pucBA Model Reactions]]
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-[[Image:pucBAModelTestSim.jpg|500px]]
+[[Image:pucBAModelTestSim.jpg|450px]]
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