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Team:Chiba/Notebook/Calendar/30 September 2009
From 2009.igem.org
(→Digestion) |
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13:20 | 13:20 | ||
| - | + | into ethyl bromide | |
13:35 | 13:35 | ||
| - | Took a picture and | + | Took a picture and cut the gel. |
| - | + | ||
| - | + | ||
| + | Then we added 2 mL of ADB Buffer and kept it at 37 degrees Celsius. | ||
=== DNA Clean & Concentrator === | === DNA Clean & Concentrator === | ||
Revision as of 23:22, 21 October 2009
(29_September_2009 <|>1_October_2009)
Contents |
Transformation
Yesterday's operation is here.
- Today's operation
10:30-
Culture at 37 degrees Celsius
20:00-
Mini Prep.
20:40-
Digestion Test
20:56-
At 37 degrees Celsius
21:45-
Gel electro...(135 V, 25 min)
PCR
Yesterday's operation is here.
11:15-
Gel electro...(135 V, 27 min)
12:10
Took a picture
Cloning
Digestion
Yesterday's operation is here.
12:37-
Gel electro...(100 V, 40 min)
13:20
into ethyl bromide
13:35
Took a picture and cut the gel.
Then we added 2 mL of ADB Buffer and kept it at 37 degrees Celsius.
DNA Clean & Concentrator
We 溶出ed 50 μL.
SAP処理
- まぜ表
15:45-
インキュベート it at 37 degrees Celsius.
---30 min---
16:20
We transferred it at 65 degrees Celsius and 失活させた
---15 min---
DNA Clean & Concentrator
We 溶出ed 20 μL.
Gel electro...
17:32
Electro...
18:15
Took a picture
Ligation
18:30
@Room Temperture
21:30
Transformation
21:50
@37 degrees Celsius
23:10
Plating
