Team:IBB Pune/project/Results

From 2009.igem.org

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==Native Biobricks==
==Native Biobricks==
-
1) pLL promoter              - BBa_I746365
+
# pLL promoter              - BBa_I746365
-
2) GFP coding sequence        - BBa_E0040
+
# GFP coding sequence        - BBa_E0040
-
3) RFP coding sequence  
+
# RFP coding sequence  
-
4) GFP Translational unit  
+
# GFP Translational unit  
-
5) RFP Translational unit  
+
# RFP Translational unit  
-
6) Enhanced CFP
+
# Enhanced CFP
-
7) Fast GFP
+
# Fast GFP
-
8) Constitutive promoter      -BBa_J23102
+
# Constitutive promoter      -BBa_J23102
-
9) Promoter + RBS
+
# Promoter + RBS
-
10) T7 promoter
+
# T7 promoter
-
11) pLuxR
+
# pLuxR
-
12) Double terminator
+
# Double terminator
-
13) RBS
+
# RBS
-
14) Constitutive LuxI
+
# Constitutive LuxI
-
15) LuxR Translational unit
+
# LuxR Translational unit
==OUR PARTS==
==OUR PARTS==
-
1) YcdB
+
# YcdB
-
2) TorA
+
# TorA
-
3) Lac Operator
+
# Lac Operator
==Assembly==
==Assembly==
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1) Const. Promoter-LacO
+
# Const. Promoter-LacO
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2) PluxR- LuxI
+
# PluxR- LuxI
-
3) {Promoter + RBS}-GFP
+
# {Promoter + RBS}-GFP
-
4) {Promoter + RBS}-TorA
+
# {Promoter + RBS}-TorA
-
5) {Promoter + RBS}-Ycdb
+
# {Promoter + RBS}-Ycdb
-
6) luxR-PluxR-{RBS-LuxI}
+
# luxR-PluxR-{RBS-LuxI}
-
7) Promoter + RBS  
+
# Promoter + RBS  
-
8) YcdB-GFP
+
# YcdB-GFP
-
9) TorA-GFP
+
# TorA-GFP
-
10) {Promoter + RBS}-LuxR
+
# {Promoter + RBS}-LuxR
-
11) {Promoter + RBS}-YcdB-GFP
+
# {Promoter + RBS}-YcdB-GFP
-
12) {Promoter + RBS}-TorA-GFP
+
# {Promoter + RBS}-TorA-GFP
-
13) Const. Promoter-LuxR
+
# Const. Promoter-LuxR
-
14)
+
#

Revision as of 00:29, 22 October 2009





We carried out the following transformations successfully:

Native Biobricks

  1. pLL promoter - BBa_I746365
  2. GFP coding sequence - BBa_E0040
  3. RFP coding sequence
  4. GFP Translational unit
  5. RFP Translational unit
  6. Enhanced CFP
  7. Fast GFP
  8. Constitutive promoter -BBa_J23102
  9. Promoter + RBS
  10. T7 promoter
  11. pLuxR
  12. Double terminator
  13. RBS
  14. Constitutive LuxI
  15. LuxR Translational unit

OUR PARTS

  1. YcdB
  2. TorA
  3. Lac Operator


Assembly

  1. Const. Promoter-LacO
  2. PluxR- LuxI
  3. {Promoter + RBS}-GFP
  4. {Promoter + RBS}-TorA
  5. {Promoter + RBS}-Ycdb
  6. luxR-PluxR-{RBS-LuxI}
  7. Promoter + RBS
  8. YcdB-GFP
  9. TorA-GFP
  10. {Promoter + RBS}-LuxR
  11. {Promoter + RBS}-YcdB-GFP
  12. {Promoter + RBS}-TorA-GFP
  13. Const. Promoter-LuxR
Retrieved from "http://2009.igem.org/Team:IBB_Pune/project/Results"