Team:University of Alberta/14 July 2009

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(New page: Reboot and Recomb: *PCR'd mCh.1 and mCh.2 using gradient PCR - FAILED *Made overnights of pKD46 in MG1655 for making electrocompetent cells *Checked miniprepped mCherry (cut with XbaI) fo...)
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Reboot and Recomb:
Reboot and Recomb:

Revision as of 00:58, 22 October 2009

University of Alberta - BioBytes










































































































Reboot and Recomb:

  • PCR'd mCh.1 and mCh.2 using gradient PCR - FAILED
  • Made overnights of pKD46 in MG1655 for making electrocompetent cells
  • Checked miniprepped mCherry (cut with XbaI) for validity
  • Colony PCR'd orn-pBA to test if ligation worked
Retrieved from "http://2009.igem.org/Team:University_of_Alberta/14_July_2009"