Team:UAB-Barcelona/CS1

From 2009.igem.org

(Difference between revisions)
(→Cloning strategy)
(→2nd round)
Line 36: Line 36:
''Biobrick 4'' (X,P) + '''RBS-GFP-T-T''' BBa_I13504 (S,P) // ''Biobrick 11''
''Biobrick 4'' (X,P) + '''RBS-GFP-T-T''' BBa_I13504 (S,P) // ''Biobrick 11''
-
''Biobrick 7'' (X,P) + '''RBS-GFP-T-T''' BBa_E0840 (S,P) // ''Biobrick 12''
+
''Biobrick 7'' (X,P) + '''RBS-RFP-T-T''' BBa_E0840 (S,P) // ''Biobrick 12''
-
 
+
====3rd round====
====3rd round====

Revision as of 01:52, 22 October 2009

Encap raro.jpg Documento sin título

Contents

Cloning strategy

1st round

phoa (E,S) + backbone BBa_K093005 (E,S) // Biobrick 1

nirk (E,S) + backbone BBa_K093005 (E,S) // Biobrick 2

nsr (X,P) + backbone BBa_K093005 (E,S) // Biobrick 3


phoa (E,S) + RBS-LacI BBa_J24679 (E,X) // Biobrick 4

pLacI-RBS-RFP-T-T BBa_J04450 (E,S) + linker BBa_B0040 (E,X) // Biobrick 5

ptet-RBS-RFP-T-T BBa_I13521 (E,S) + linker BBa_B0040 (E,X) // Biobrick 6


nirk (E,S) + RBS-LacI BBa_J24679 (E,X) // Biobrick 7

RBS BBa_J61127 (S,P) + nsr (X,P) // Biobrick 8

ptet BBa_R0040 (S,P) + Biobrick 8 (X,P) // Biobrick 9

placI BBa_K091112 (S,P) + Biobrick 8 (X,P) // Biobrick 10


2nd round

Biobrick 4 (X,P) + RBS-GFP-T-T BBa_I13504 (S,P) // Biobrick 11

Biobrick 7 (X,P) + RBS-RFP-T-T BBa_E0840 (S,P) // Biobrick 12

3rd round

Biobrick 11 + Biobrick 5 // Biobrick 13

Biobrick 11 + Biobrick 6 // Biobrick 14

Biobrick 9 (X,P) + RBS-GFP-T-T BBa_I13504 (S,P) // Biobrick 15

Biobrick 10 (X,P) + RBS-GFP-T-T BBa_I13504 (S,P) // Biobrick 16


Biobricks used

BBa_R0040 http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 ptet P1 6I

BBa_J04450 http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 pLacI-RBS-RFP-T-T P1 7A

BBa_J24679 http://partsregistry.org/wiki/index.php?title=Part:BBa_J24679 RBS-LacI P1 7L

BBa_J61127 http://partsregistry.org/wiki/index.php?title=Part:BBa_J61127 RBS P1 11N

BBa_E0840 http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840 RBS-GFP-T-T P1 12O

BBa_P0140 http://partsregistry.org/wiki/index.php?title=Part:BBa_P0140 RBS-tetR P2 1F

BBa_P0340 http://partsregistry.org/wiki/index.php?title=Part:BBa_P0340 RBS-tetR P2 1J

BBa_K091112 http://partsregistry.org/wiki/index.php?title=Part:BBa_K091112 placI P2 8B

BBa_K093005 http://partsregistry.org/wiki/index.php?title=Part:BBa_K093005 RBS-RFP-T-T P3 13K

Retrieved from "http://2009.igem.org/Team:UAB-Barcelona/CS1"