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Team:CityColSanFrancisco/Material
From 2009.igem.org
(Difference between revisions)
(New page: == '''Bacteria''' == '''Chemotropes which reduce Fe3:''' *'''S. Oneidensis ''' *'''R. ferireducines ''' '''Phototropes which Oxidize Fe2+ ''' *'''R. palustris – hopefully transformed!...) |
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'''Genetic manipulation ''' | '''Genetic manipulation ''' | ||
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*'''P. aeruginosa- source of OprB gene (membrane porin sequence).''' | *'''P. aeruginosa- source of OprB gene (membrane porin sequence).''' | ||
*'''pJQ200SK plasmid suicide vector for R. palustris. ''' | *'''pJQ200SK plasmid suicide vector for R. palustris. ''' | ||
*'''E. coli- for plasmid production/ purification. ''' | *'''E. coli- for plasmid production/ purification. ''' | ||
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| + | == '''Primers for isolating and manipulating genes''' == | ||
| + | Pseudomonas aeruginosa OprB primer | ||
| + | Forward: 5' ATGTACAAGAACAAGAAAACC 3' | ||
| + | Reverse: 5' TCAGAACACCGTCTGGATC 3' | ||
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| + | Mutegen primer to change GCTGCA to GCAGCA in OprB gene so it can be a biobrick | ||
| + | Forward: 5' CTGTACCAGCCGCaGCAGGACACCGAG 3' | ||
| + | Reverse: 5' CTCGGTGTCCTGCtGCGGCTGGTACAG 3' | ||
Revision as of 02:14, 22 October 2009
Bacteria
Chemotropes which reduce Fe3:
- S. Oneidensis
- R. ferireducines
Phototropes which Oxidize Fe2+
- R. palustris – hopefully transformed!
Genetic manipulation
- P. aeruginosa- source of OprB gene (membrane porin sequence).
- pJQ200SK plasmid suicide vector for R. palustris.
- E. coli- for plasmid production/ purification.
Primers for isolating and manipulating genes
Pseudomonas aeruginosa OprB primer
Forward: 5' ATGTACAAGAACAAGAAAACC 3' Reverse: 5' TCAGAACACCGTCTGGATC 3'
Mutegen primer to change GCTGCA to GCAGCA in OprB gene so it can be a biobrick
Forward: 5' CTGTACCAGCCGCaGCAGGACACCGAG 3' Reverse: 5' CTCGGTGTCCTGCtGCGGCTGGTACAG 3'
Battery
