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Team:SDU-Denmark/Notebook
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'''Task:''' Find a backbone for both e.coli and b. subtilis | '''Task:''' Find a backbone for both e.coli and b. subtilis | ||
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'''Status:''' Doing. | '''Status:''' Doing. | ||
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'''People:''' Helle and Mike | '''People:''' Helle and Mike | ||
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'''Last edit:''' July 8th 2009. | '''Last edit:''' July 8th 2009. | ||
Revision as of 12:36, 8 July 2009
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook |
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Contents |
Project task-management notebook
Week 1 - BioBricks, primers, protocols and more
Plasmid backbone
Task: Find a backbone for both e.coli and b. subtilis
Status: Doing.
People: Helle and Mike
Last edit: July 8th 2009.
July 7th 2009:
We found the backbone, [http://partsregistry.org/wiki/index.php/Part:BBa_I742123 BBa_I742123], which other teams have found to be compatible with both both gram positive and gram negative bacteria, such as e. coli.
After talking to HQ, it turns out that the quality of their stock is bad, and they will try to get some home from older teams. Furthermore, it might simply be better to use a more specifik backbone for e. coli and for b. subtilis.
(mike)
July 8th 2009:
We decided to start working with [http://partsregistry.org/Part:pSB1A3 pSB1A3] instead, which is a high-output e. coli backbone.
Output: 100-300 number pr. cell. Resistance: ampicillin (we're going with 50 µg/mL first)
(mike)
Inducible promoter
7.July Anna
I found the inducible promoter BBa_R0011 that is regulated by LacI.
It is a strong promoter, that will be on in stains without LacI.
E. coli strain
The plasmid seems to be working in most types (no need for ccdB) Jacob suggests E. coli KG22, which can turns on the operon by adding IPTG.
