"
Team:EPF-Lausanne/Notebook/Wet Lab
From 2009.igem.org
(Difference between revisions)
Nbranden (Talk | contribs)
(New page: =Wet Lab= ==06.07.09== LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli following received protocol, and grown overnight (see Lab book for more details). <br>One probl...)
Newer edit →
(New page: =Wet Lab= ==06.07.09== LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli following received protocol, and grown overnight (see Lab book for more details). <br>One probl...)
Newer edit →
Revision as of 13:59, 8 July 2009
Wet Lab
06.07.09
LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli following received protocol, and grown overnight (see Lab book for more details).
One problem: we actually don't know TrpR plasmid resistance, so we tried with three resistances available in the lab: Amp., Kana. and Chl.
LOVTAP is in a plasmid called pCal-n (see picture below):
Some comments on the plasmid:
-CBP is a small peptide with which we could purify LOVTAP protein
-Thrombin target is a nucleotidic sequence that can be recognized by thrombin a peptidase. This peptidase will cut CBP once LOVTAP is purified
