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| Oligo Name | Ordered by... | Length | Sequence | Description | ||
| Pimer forward CAT | Sigma | 64 | GATCGCAATTGACCAACAAGGAGAAATCTAGATGGCCGGCGAGAAAAAAATCACTGGATATACC | designed to get CAT-gene + Freigem restriction sites out of the lab vector no 258 | ||
| Primer reverse CAT | Sigma | 58 | GTGGCAGGGCGGGGCGACCGGTTAATACTAGTAGCGGCCGCCTGCAGAAGCTTGGGTA | designed to get CAT-gene + Freigem restriction sites out of the lab vector no 258 | ||
| Short Linker | Sigma | 18 | CTAGAGGTGGTTCTGGTA | GlySerGlyGly | ||
| Middle Linker | Sigma | 30 | CTAGAGGTGGTTCTGGTACTAGAGGTGGTTCTGGTA | GlySerGlyGlyx2 | ||
| Long Linker | Sigma | 42 | CTAGAGGTGGTTCTGGTACTAGAGGTGGTTCTGGTACTAGAGGTGGTTCTGGTA | GlySerGlyGlyx3 | ||
| Primer forward GenIII | Sigma | 52 | GAATTCGCGGCCGCTTCTAGATGGCCGGCGATTTTGATTATGAAAAGATGGC | Insert of GenIII(restriction sites RFC25) into M13DNA , | ||
| Primer reverse GenIII | Sigma | 53 | CTGCAGGCGGCCGCTACTAGTATTAACCGGTAGACTCCTTATTACGCAGTATG | Insert of GenIII(restriction sites RFC25) into M13DNA , | ||
| A4 AGO | Sigma | 21 | AAGTTTTTTGGGGTCGAGGTG | guide oligo complentary to target sequenz, the cutting event will be done after the tenth base 5'3' | ||
| A1 AGO | Sigma | 21 | ACAACCATCGCCCACGCATAA | guide oligo complentary to target sequenz, the cutting event will be done after the tenth base 5'3'.The M13ssDNA will be cut at position 3100 | ||
| A2 AGO | Sigma | 21 | GGTTTTACTCTGATTCTCTTC | guide oligo complentary to target sequenz, the cutting event will be done after the tenth base of the oligo 5'3'.The M13ssDNA will be cut at position 550. | ||
| A3 AGO | Sigma | 21 | TACCTTCGGGTACTCTTCTAA | guide oligo complentary to target sequenz, the cutting event will be done after the tenth base 5'3'.The M13ssDNA will be cut at position 1280. | ||
| A5_Ago_pETfragment | Sigma | 21 | AAGTTTTTTGGGGTCGAGGTG | guide oligo complentary to target sequenz, the cutting event will be done after the tenth base 5'3'.The M13ssDNA will be cut at position 100 | ||
| Aa BB Primer Middle a | Sigma | 50 | TCGTTCATTTTGAAATCCCCTGAACTCTTCTTCACCCACTCTTTTCAGTT | deleted EcoRI site | ||
| Aa BB Primer Middle b | Sigma | 50 | GGAAGGTGATATTATGTACTGGCTTTAATAATACTAGTAGCGGCCGCTGCAG | deleted EcoRI site | ||
| Aa BB Primer Start | Sigma | 45 | GGTTTAAAAGAGCTTCCTTTCCCATCTAGAAGCGGCCGCGAATTC | Addon Tail to add Biobrick prefix | ||
| Aa BB Primer Back | Sigma | 52 | AACTGAAAAGAGTGGGTGAAGAAGAGTTCAGGGGATTTCAAAATGAACGA | Addon Tail to add Biobrick suffix | ||
| AGO-target _M13 | Sigma | 60 | CTGCAAGCCTCAGCGACCGAATATATCGGTTATGCGTGGGCGATGGTTGTTGTCATTGTC | 5'biotinylated olgio for phage display | ||
| AGO-guide-A1 5' Phos | Sigma | 21 | [Phos]ACAACCATCGCCCACGCATAA | phosporilised(5') for phage display assay with AGO protein | ||
| AGO-guide-A4 5' Phos | Sigma | 21 | [Phos]AAGTTTTTTGGGGTCGAGGTG | phosporilised(5') for phage display assay with AGO protein | ||
| Fok control 1 | Sigma | 40 | TTCTACTAATAGTAGTAGCATTAACATCCAATAAATCATA | 1669bp for the bond of gel elctrophoresis , the oligo hybridisized to the ssDNA enable Fok to cut | ||
| Fok control 2 | Sigma | 40 | GTCATTTTTGCGGATGGCTTAGAGCTTAATTGCTGAATAT | 1914bp for the bond of gel elctrophoresis, the oligo hybridisized to the ssDNA enable Fok to cut | ||
| Fok control 3 | Sigma | 40 | TGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGT | 786bp for the bond of gel elctrophoresis,the oligo hybridisized to the ssDNA enable Fok to cut | ||
| 5'fluo40bp | Sigma | 40 | Test nucleotide for the in vivo assay | |||
| xbaI_RBS-Strep-Fok_a | Sigma | 40 | GCTCTAGAGAAGGAGATATACTATGGCCGGCTGGAGCCAT | Insert a RBS befor the StrepTag | ||
| StrepFoka_r | Sigma | 28 | GAAGCTTCTGCAGCGGCCGCTACTAGTA | Insert a RBS befor the StrepTag | ||
| XbaI_DsbAss_AgeI_2r | Sigma | 62 | CCGGTCGCCGATGCGCTAAACGCTAAAACTAAACCAGCCAGCGCCAGCCAAATCTTTTTCAT | olgio with xbaI and ageI ends , can linked on DsbA signalsequenz | ||
| pBAD-TOPO f | Sigma | 34 | GTCCCCCCGGGAACCCCGCTTATTAAAAGCATTC | add-on tail primer for pcr to remove the two AgeI sites of the vector and to introduce the bb-pre and suffix | ||
| pBAD-TOPO r | Sigma | 56 | TCCCCCCGGGCTGCAGTATGAATTCACTCCTTCTTAAAGTTAAACAAAATTATTTC | add-on tail primer for pcr to remove the two AgeI sites of the vector and to introduce the bb-pre and suffix | ||
| XbaI_NgoMIV_DsbA forward | Sigma | 19 | CGCTTCTAGATGGCCGGCA | add-on tail primer to generte a FOS-DsbA-Split-Fok_a part | ||
| DsbA-Fos | Sigma | 36 | CGTTTAGCGCATCGGCGCACCATCACCACCACCATG | add-on tail primer to generte a FOS-DsbA-Split-Fok_a part | ||
| Fos-SplitFok_a | Sigma | 38 | GGAGTTCATCCTGGCAGCACGACCAGCCTGTAAGATTC | add-on tail primer to generte a FOS-DsbA-Split-Fok_a part | ||
| SplitFok_a age_Spe_not_pst | Sigma | 17 | AGCTCTGCAGCGGCCGC | add-on tail primer to generte a FOS-DsbA-Split-Fok_a part | ||
| 80mer Fok control | Sigma | 80 | AGTTCGGTTCCCTTATGATTGACCGTCTGCGCCTCGTTCCGGCTAAGTAACATGGAGCAGGTCGCGGATTTCGACACAAT | target oligonucleotide for Fok cleavage assay | ||
| 80mer Fok control | Sigma | 80 | [Cy3]AGTTCGGTTCCCTTATGATTGACCGTCTGCGCCTCGTTCCGGCTAAGTAACATGGAGCAGGTCGCGGATTTCGACACAAT | target oligonucleotide for Fok cleavage assay with fluorescine mark | ||
| Standard 35 forward | Sigma | 25 | CCGAATTCGCGGCCGCTTCTAGATG | freiburg stadard primer (standard 25) to amplify insert | ||
| Standard 35 reverse | Sigma | 29 | GCTCTGCAGCGGCCGCTACTAGTATTAAC | freiburg stadard primer (standard 25) to amplify insert | ||
| pr_fwd_Xba_SDII_TorA | Sigma | 55 | ATATAAATTCTAGATAACGAGGGCAAATCATGAACAATAACGATCTCTTTCAGGC | Shine-Dalgarno sequence II TorA(signal sequence for periplasm transport) | ||
| ssDNA_prod_pET39b+ | Sigma | 23 | CGGATCCGATATCGCCATGGTTG | primer for modifies thermocycler protocoll to produce ssDNA | ||
| fokm_li_xbaI_ageI | Sigma | 90 | CTAGATGGCCGGCGGTTCTGGTGGTGGTTCTGGCGGTGGTTCTGGAGGTAGTTCTGGCGGTGGATCTGGAGGCGGTTCTGGGTCAGGATC | 36 GS linker wrong | ||
| fok_m_link_NgoMIV_HINFI_compl | Sigma | 91 | CCGGCGGTGGTTCTGGTGGTGGTTCTGGCGGTGGTTCTGGAGGTAGTTCTGGCGGTGGATCTGGAGGCGGTTCTGGGTCAGGATCTGGTGATGGTTCTGGCTCTGGG | 36 Gs linker wrong complement | ||
| o_diA1_5dig_site1 | Purimex | 16 | CGGAACGAGGCGCAGA | Modified oligonucleotid to guide the Fok-Complex to the target sequence via digoxigenin | ||
| o_diAB1_5fluo_site1 | Purimex | 16 | CGGTCAATCATAAGGG | Modified oligonucleotid to guide the Fok-Complex to the target sequence via fluorescin | ||
| o_diB1_15dig_site1 | Purimex | 30 | CCATGTTACTTAGCCGGAACGAGGCGCAGA | Modified oligonucleotid to guide the Fok-Complex to the target sequence via digoxigenin | ||
| o_mono1_3fluo_14dig_site1 | Purimex | 30 | CATGTTACTTAGCCGGAACGAGGCGCAGAC | Modified oligonucleotid to guide the Fok-Complex to the target sequence via digoxigenin and fluorecine |
Team:Freiburg bioware/oligos
From 2009.igem.org
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Latest revision as of 02:40, 22 October 2009
