Team:IBB Pune/project/Results

From 2009.igem.org

Revision as of 02:43, 22 October 2009

Characterization of And Gates:

Even as the wiki freezes we are characterizing Const.Prom-LacO-mRFP for its expression.

We carried out the following transformations successfully:

Native Biobricks

1. pLL promoter - BBa_I746365
2. GFP coding sequence - BBa_E0040
3. RFP coding sequence
4. GFP Translational unit
5. RFP Translational unit
6. Enhanced CFP
7. Fast GFP
8. Constitutive promoter -BBa_J23102
9. Promoter + RBS
10. T7 promoter
11. pLuxR
12. Double terminator
13. RBS
14. Constitutive LuxI
15. LuxR Translational unit

OUR PARTS

1. YcdB
2. TorA
3. Lac Operator

Assembly

1. Const. Promoter-LacO
2. PluxR- LuxI
3. {Promoter + RBS}-GFP
4. {Promoter + RBS}-TorA
5. {Promoter + RBS}-Ycdb
6. luxR-PluxR-{RBS-LuxI}
7. Promoter + RBS
8. YcdB-GFP
9. TorA-GFP
10. {Promoter + RBS}-LuxR
11. {Promoter + RBS}-YcdB-GFP
12. {Promoter + RBS}-TorA-GFP
13. Const. Promoter-LuxR

Failed Transformations

Backbones

1. pSB1AK3 with CcdB gene
2. pSB1T3 with CcdB gene
3. pSB1C3 with CcdB gene
4. pSB1K3 with CcdB gene
5. pSB1A3 with CcdB gene
6. pSB1AC3 with CcdB gene
7. pSB1AK3 with mRFP
8. pSB1T3 with mRFP
9. pSB1C3 with mRFP
10. pSB1K3 with mRFP
11. pSB1A3 with mRFP
12. pSB1AC3 with mRFP

Native Biobricks

1. Lysis Cassette
2. Constitutive GFP
3. COnstitutive CFP

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