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General Activities
Characterize or improve an existing BioBrick Part or
Device and enter this information back on the Registry.
We corrected the sequences of the existing basic parts: BBa_K157004,BBa_K157009,BBa_K157011
and BBa_K157012.
The team Freiburg08 had entered the sequence information with
the pre- and suffix sequences from RFC25
into the registry, which means they add the nucleotides sequence for
the restriction site for and after the part information. This caused an
incompatibility in the compatibility check for RFC25. Since the RFC25
is now an officially accepted Registry standard for Biobricks, we
deleted the pre and suffix sequences, to use these parts as basic parts
for our composite parts. This prevents that the sequence information of
the pre and suffix causes a faulty sequence when fused automatically by
the registry.
Develop and document a new technical standard that supports
the:
design of BioBrick Parts or Devices,
or construction of BioBrick Parts or Devices
In addition to this,we recognized that there was no automated way to
fuse basic parts, designed according to the RFC25.
The automatic fusion program of the registry is only able to fuse basic
parts with the normal Biobrick standard, or it gives the option to fuse
basic parts blunt end without the Biobrick scar. However, parts
according to the RFC25 produce a different scar compared those of the
normal Biobrick standard. The difference between the two scars would
produce a difference between the theoretical sequence of composite
parts and the real sequence when the parts are fused according to RFC25
in the wet lab. Therefore, we looked for a solution to fuse our basic
parts in the right way in silico, so that the sequence in the part
registry is correct. We first had the idea to modify the sequence
manually by adding the RFC25 scar to our basic parts, but
asked Randy Rettberg also for a better solution. He designed the part BBa_B0105, which has the sequence
information of the RFC25 scar. With this, it is
possible now to produce composite parts with RFC25 basic parts by inserting the
part BBa_B0105
between the basic parts.
For example, a fusion of the parts BBa_K157011, BBa_K243003,
BBa_K157009 according to the RFC25 standard would need to have the
above mentioned BBa_B0105 part inserted between all
other parts to generate the following input:
When you follow these instructions for all parts designed according to RFC25 the produced sequence is
correct.
