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Team:CityColSanFrancisco/Material
From 2009.igem.org
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'''Phototropes which Oxidize Fe2+ ''' | '''Phototropes which Oxidize Fe2+ ''' | ||
| - | *'''R. palustris – hopefully transformed!''' | + | *'''R. palustris TIE-1 strain – hopefully transformed!''' |
'''Genetic manipulation ''' | '''Genetic manipulation ''' | ||
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Reverse: 5' CTCGGTGTCCTGCtGCGGCTGGTACAG 3' | Reverse: 5' CTCGGTGTCCTGCtGCGGCTGGTACAG 3' | ||
| + | R. palustris OprB primer -R. palustris might be upregulated | ||
| + | Forward: 5' ATGCTGCCGTCGCGCTGGCT 3' | ||
| + | Reverse: 5' TCAGAACTGCGTGCGCAGTCG 3' | ||
== '''Battery''' == | == '''Battery''' == | ||
[[Image:Fuel_Cell_Conceptualization.png|500px]] | [[Image:Fuel_Cell_Conceptualization.png|500px]] | ||
Latest revision as of 03:56, 22 October 2009
Bacteria
Chemotropes which reduce Fe3:
- S. Oneidensis
- R. ferireducines
Phototropes which Oxidize Fe2+
- R. palustris TIE-1 strain – hopefully transformed!
Genetic manipulation
- P. aeruginosa- source of OprB gene[1] (membrane porin sequence).
- pJQ200SK plasmid suicide vector for R. palustris.
- E. coli- for plasmid production/ purification.
Primers for isolating and manipulating genes
Pseudomonas aeruginosa OprB primer
Forward: 5' ATGTACAAGAACAAGAAAACC 3' Reverse: 5' TCAGAACACCGTCTGGATC 3'
Mutegen primer to change GCTGCA to GCAGCA in OprB gene so it can be a biobrick
Forward: 5' CTGTACCAGCCGCaGCAGGACACCGAG 3' Reverse: 5' CTCGGTGTCCTGCtGCGGCTGGTACAG 3'
R. palustris OprB primer -R. palustris might be upregulated
Forward: 5' ATGCTGCCGTCGCGCTGGCT 3' Reverse: 5' TCAGAACTGCGTGCGCAGTCG 3'
Battery
