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UCL London/Protocol/Glycerol Stock
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(New page: '''Glycerol Stock''' :'''''Materials:''''' * Glycerol * Transformed E.coli cells. : '''''Method:''''' # Add 80µL glycerol into each labelled eppendorf. # Add 300µL E.coli suspension...)
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(New page: '''Glycerol Stock''' :'''''Materials:''''' * Glycerol * Transformed E.coli cells. : '''''Method:''''' # Add 80µL glycerol into each labelled eppendorf. # Add 300µL E.coli suspension...)
Newer edit →
Revision as of 11:57, 9 July 2009
Glycerol Stock
- Materials:
- Glycerol
- Transformed E.coli cells.
- Method:
- Add 80µL glycerol into each labelled eppendorf.
- Add 300µL E.coli suspension into the glycerol.
- Vortex & store at -20°C.
- Rest of the E.coli suspension are stored in the fridge.
