Team:UNC Chapel Hill/Project Needs

From 2009.igem.org

(Difference between revisions)
(→Basic Lab Supplies)
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==Basic Lab Supplies==
==Basic Lab Supplies==
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# Petri Dishes
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# Blank Petri Dishes
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# Gloves
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# Disposable Gloves (S, M, L)
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# Screw Top Microcentrifuge Tubes
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# Screw Top Microcentrifuge Tubes (1.5 mL)
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# Cell Culture Tubes
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# Cell Culture Tubes (10 mL)
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# Eppendorf Tubes
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# Eppendorf Tubes (1.5 mL)
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# 9 mL glass Pasteur pipets
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# Glass Pasteur pipets (9 mL)
# Parafilm
# Parafilm
# Micropipets (P2, P20, P200, P1000)
# Micropipets (P2, P20, P200, P1000)
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# Micropipet Tips
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# Micropipet Tips (for all sizes listed above)
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# Restriction Enzymes-if from Fermentas, should be FastDigest versions.  If NEB, we need EcoRI-HF.
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# Restriction Enzymes-if from Fermentas, should be FastDigest versions.  If NEB, we need EcoRI-HF:
## EcoRI
## EcoRI
## Xbal
## Xbal
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# T4 DNA Ligase
# T4 DNA Ligase
# Agarose Powder
# Agarose Powder
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# Luria Broth
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# Luria Broth and/or Super Optimal Broth
# Buffer for Gel Electrophoresis
# Buffer for Gel Electrophoresis
# Antibiotics:
# Antibiotics:

Revision as of 14:13, 10 July 2009

Basic Lab Supplies

  1. Blank Petri Dishes
  2. Disposable Gloves (S, M, L)
  3. Screw Top Microcentrifuge Tubes (1.5 mL)
  4. Cell Culture Tubes (10 mL)
  5. Eppendorf Tubes (1.5 mL)
  6. Glass Pasteur pipets (9 mL)
  7. Parafilm
  8. Micropipets (P2, P20, P200, P1000)
  9. Micropipet Tips (for all sizes listed above)
  10. Restriction Enzymes-if from Fermentas, should be FastDigest versions. If NEB, we need EcoRI-HF:
    1. EcoRI
    2. Xbal
    3. SpeI
    4. PstI
    5. MunI (MfeI)
    6. Mph1103I (NsiI)
  11. T4 DNA Ligase
  12. Agarose Powder
  13. Luria Broth and/or Super Optimal Broth
  14. Buffer for Gel Electrophoresis
  15. Antibiotics:
    1. Carbenicillin
    2. Kanamycin
  16. Gel Extraction Kit
  17. PCR Supermix
  18. Competent Cells (DH10B, Top10, or DH5a. DB3.1)

Equipment

  1. Thermal Cycler
  2. Fluorometer

Other

  1. Plane tickets to Boston for Jamboree weekend (October 31 to November 2nd, 2009)
  2. Hotel stay in Boston for Jamboree
  3. Attendance fee for Jamboree ($175 per student; $375 per advisor)
  4. Custom T-shirts
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