Team:Illinois/Hybrid Promoter

From 2009.igem.org

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(July 10)
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== '''July 10''' ==
== '''July 10''' ==
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Today we looked more closely at the biobricks: BBa_I14032, BBa_R0040, BBa_K137125, BBa_K091101.
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Today we came up with a schematic of a possible decoder: [[Image:IllinoisDecoderjuly10.png|200px|thumb|center]]
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We looked more closely at the biobricks: BBa_I14032, BBa_R0040, BBa_K137125, BBa_K091101.
BBa_R0040 and BBa_K091101 were available to us in the registry distribution so we transformed these into cells to test them.
BBa_R0040 and BBa_K091101 were available to us in the registry distribution so we transformed these into cells to test them.

Revision as of 20:38, 10 July 2009

Click to go to the Illinois home page




Hybrid Promoter

Goals: The goal of this side-project is the create a hybrid or combinatorial promoter that accepts two inputs. This will be used in the creation of an AND logic gate.


Papers of Interest:

[http://www.nature.com/nature/journal/v420/n6912/full/nature01257.html Engineered gene circuits] Jeff Hasty, David McMillen & J. J. Collins

[http://aem.asm.org/cgi/content/abstract/75/3/637 Construction and Enhancement of a Minimal Genetic AND Logic Gate] Daniel J. Sayut, Yan Niu, and Lianhong Sun

[http://www.sciencemag.org/cgi/content/full/296/5572/1466 Combinatorial Synthesis of Genetic Networks] Cabrevelin C. Guet, Michael B. Elowitz, Weihong Hsing, Stanislas Leibler

[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2132448 Programming gene expression with combinatorial promoters] Robert Sidney Cox, III, Michael G Surette, and Michael B Elowitz


Biobricks of Interest:

BBa_K091101 pTet_Lac hybrid promoter

BBa_R0010 lacl regulated promoter

BBa_R0040 TetR repressible promoter

BBa_K137125 LacI-repressed promoter B4

July 10

Today we came up with a schematic of a possible decoder:
IllinoisDecoderjuly10.png


We looked more closely at the biobricks: BBa_I14032, BBa_R0040, BBa_K137125, BBa_K091101.

BBa_R0040 and BBa_K091101 were available to us in the registry distribution so we transformed these into cells to test them.