Team:UNIPV-Pavia/Freezer

From 2009.igem.org

(Difference between revisions)
Line 298: Line 298:
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a></td>
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a></td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A3</td>
+
  <tr class="in_freezer"><td class="prova">A3-1</td>
<td class="prova">I732017-B0015<br>(RBS-lacZ-TT)</td>
<td class="prova">I732017-B0015<br>(RBS-lacZ-TT)</td>
                 <td class="prova">Jun 12</td>
                 <td class="prova">Jun 12</td>
-
<td class="prova">1</td>
+
<td class="prova">2</td>
<td class="prova">TOP10</td>
<td class="prova">TOP10</td>
<td class="prova">pSB1AK3</td>
<td class="prova">pSB1AK3</td>
Line 309: Line 309:
<td class="prova">P0440-R0040<br>(RBS-tetR-TT-Ptet)</td>
<td class="prova">P0440-R0040<br>(RBS-tetR-TT-Ptet)</td>
                 <td class="prova">Jun 11</td>
                 <td class="prova">Jun 11</td>
-
<td class="prova">1</td>
+
<td class="prova">2</td>
<td class="prova">TOP10</td>
<td class="prova">TOP10</td>
<td class="prova">pSB1A2</td>
<td class="prova">pSB1A2</td>
Line 378: Line 378:
<td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9.jpg">(digestion)</a> but works</td>
<td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9.jpg">(digestion)</a> but works</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A10</td>
+
  <tr class="in_freezer"><td class="prova">A10-thrown away</td>
<td class="prova">K117000-B0015<br>(celB-TT)</td>
<td class="prova">K117000-B0015<br>(celB-TT)</td>
                 <td class="prova">Jun 30</td>
                 <td class="prova">Jun 30</td>
Line 405: Line 405:
<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td>
<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td>
                 <td class="prova">Jul 10</td>
                 <td class="prova">Jul 10</td>
-
<td class="prova">0</td>
+
<td class="prova">1</td>
<td class="prova">TOP10</td>
<td class="prova">TOP10</td>
<td class="prova">pSB1A2</td>
<td class="prova">pSB1A2</td>
Line 429: Line 429:
<td class="prova">Ptet-RBS-luxR-TT-Plux<br></td>
<td class="prova">Ptet-RBS-luxR-TT-Plux<br></td>
                 <td class="prova">Jul 10</td>
                 <td class="prova">Jul 10</td>
-
<td class="prova">0</td>
+
<td class="prova">1</td>
<td class="prova">TOP10</td>
<td class="prova">TOP10</td>
<td class="prova">pSB1A2</td>
<td class="prova">pSB1A2</td>

Revision as of 14:48, 13 July 2009

EthanolPVanimation.gif

Biobrick/
Ligation Name		 Description Creation Date N° usage Strain Vector Quality Control
J23101(J101) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23102(J102) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23103(J103) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23104(J104) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23105(J105) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23106(J106) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23110(J110) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23112(J112) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23113(J113) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23114(J114) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23116(J116) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23117(J117) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23118(J118) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
E0240(GFP3) GFP protein generator
(RBS-GFP-TT)		 May 25 5 TOP10 pSB1A2 length ok (PCR) sequence ok (MIT)
K131009(E2) colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS) May 28 1 TOP10 pSB1AK3 sequence BAD in prefix,suffix,celB (MIT,BMR)
K117000(celB) colE7 lysis gene May 28 1 TOP10 pSB1A2 sequence ok (MIT)
K124017(lysis21) Phage Lysis Cassette May 28 0 TOP10 pSB1AK3 sequence BAD (MIT)
B0015(TT) Double terminator May 28 2 TOP10 pSB1AK3 sequence ok (MIT)
I732017 (RBS-lacZ) RBS-lacZ May 28 1 TOP10 pSB1A2 long part (MIT)
B0030 RBS 0.6 Jun 4 0 TOP10 pSB1A2 sequence ok (MIT,BMR)
B0031 RBS 0.07 Jun 4 0 TOP10 pSB1A2 sequence ok (MIT)
B0032 RBS 0.3 Jun 4 0 TOP10 pSB1A2 sequence ok (MIT)
B0033 RBS 0.01 Jun 4 0 TOP10 pSB1A2 sequence ok (MIT)
B0034 RBS 1 Jun 4 0 TOP10 pSB1A2 sequence ok (MIT)
P0440(P04) RBS-tetR-TT Jun 4 1 TOP10 pSB1A2 length ok (PCR) sequence ok (MIT)
R0040(Ptet) Ptet Jun 4 1 TOP10 pSB1A2 sequence ok (MIT)
T9002 Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT Jun 4 3 TOP10 pSB1A3 sequence BAD (MIT) - sequence ok (BMR)
J23100(J100bis) constitutive promoter Jun 4 3 TOP10 J61002(Amp) sequence ok (MIT,BMR)
J23101(J101bis) constitutive promoter Jun 4 2 TOP10 J61002(Amp) sequence ok (MIT)
J23118(J118bis) constitutive promoter Jun 4 2 TOP10 J61002(Amp) sequence ok (MIT)
A1 J23100(Jun 4)-E0240
(Pcon-RBS-GFP-TT)		 Jun 11 3 TOP10 pSB1A2 length ok (PCR)
A2 J23101(Jun 4)-E0240
(Pcon-RBS-GFP-TT)		 Jun 11 1 TOP10 pSB1A2 length ok (PCR)
A3-1 I732017-B0015
(RBS-lacZ-TT)		 Jun 12 2 TOP10 pSB1AK3 length ok (digestion) sequence ok -long part- (BMR)
A4 P0440-R0040
(RBS-tetR-TT-Ptet)		 Jun 11 2 TOP10 pSB1A2 length ok (PCR) sequence ok (BMR)
A5 A4-A3
(RBS-tetR-TT-Ptet-RBS-lacZ-TT)		 Jun 19 0 TOP10 pSB1AK3 sequence ok -long part- (BMR)
A6 J23100(Jun 4)-A4
(Pcon-RBS-tetR-TT-Ptet)		 Jun 19 1 TOP10 pSB1A2 length ok (PCR) sequence ok (BMR)
BOL1 J23118-B0034-C0012-B0015
(Pcon-RBS-lacI-TT)		 Jun 23 1 TOP10 pSB1A2 sequence ok (BMR)
A7 J23118(Jun 4)-E0240
(Pcon-RBS-GFP-TT)		 Jun 25 1 TOP10 pSB1A2 length ok (PCR)
R0011(Plac) Plac Jun 25 0 TOP10 pSB1A2 sequence ok (MIT)
K112808(ENTERO) holin-lysozyme-T-Pconweak-antiholin-T (with RBSs) Jun 25 0 TOP10 pSB1A2 sequence ok -long part- (MIT)
A8 A4-E0240
(RBS-tetR-TT-Ptet-RBS-GFP-TT)		 Jun 29 2 TOP10 pSB1A2 NON PURE PLASMID! (digestion) but works
A9 A6-E0240
(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)		 Jun 29 2 TOP10 pSB1A2 NON PURE PLASMID! (digestion) but works
A10-thrown away K117000-B0015
(celB-TT)		 Jun 30 2 TOP10 pSB1AK3 sequence BAD - repeat ligation
A8pg A4-E0240
(RBS-tetR-TT-Ptet-RBS-GFP-TT)		 Jul 3 0 TOP10 pSB1A2 length ok (digestion)
A9pg A6-E0240
(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)		 Jul 3 2 TOP10 pSB1AK3 length ok (digestion)
A11-1 BOL1-R0011
(Pcon-RBS-lacI-TT-Plac)		 Jul 10 1 TOP10 pSB1A2 length ok (PCR)
A11-3 BOL1-R0011
(Pcon-RBS-lacI-TT-Plac)		 Jul 10 0 TOP10 pSB1A2 length ok (PCR)
A11-6 BOL1-R0011
(Pcon-RBS-lacI-TT-Plac)		 Jul 10 0 TOP10 pSB1A2 length ok (PCR)
F2620 Ptet-RBS-luxR-TT-Plux
 Jul 10 1 TOP10 pSB1A2 sequence BAD (MIT)

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