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Team:Illinois/Hybrid Promoter
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BBa_R0040 and BBa_K091101 were available to us in the registry distribution so we transformed these into cells to test them. | BBa_R0040 and BBa_K091101 were available to us in the registry distribution so we transformed these into cells to test them. | ||
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| + | == '''July 13''' == | ||
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| + | Concerns: We want the sRNA gene on a high copy plasmid and the target sequence on low copy plasmid. This could require up to 7 different plasmids to transform into the cell. We need to look into the feasibility of this. | ||
Revision as of 15:29, 13 July 2009
Hybrid Promoter
Goals: The goal of this side-project is the create a hybrid or combinatorial promoter that accepts two inputs. This will be used in the creation of an AND logic gate.
Papers of Interest:
[http://www.nature.com/nature/journal/v420/n6912/full/nature01257.html Engineered gene circuits] Jeff Hasty, David McMillen & J. J. Collins
[http://aem.asm.org/cgi/content/abstract/75/3/637 Construction and Enhancement of a Minimal Genetic AND Logic Gate] Daniel J. Sayut, Yan Niu, and Lianhong Sun
[http://www.sciencemag.org/cgi/content/full/296/5572/1466 Combinatorial Synthesis of Genetic Networks] Cabrevelin C. Guet, Michael B. Elowitz, Weihong Hsing, Stanislas Leibler
[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2132448 Programming gene expression with combinatorial promoters] Robert Sidney Cox, III, Michael G Surette, and Michael B Elowitz
Biobricks of Interest:
BBa_K091101 pTet_Lac hybrid promoter
BBa_R0010 lacl regulated promoter
BBa_R0040 TetR repressible promoter
BBa_K137125 LacI-repressed promoter B4
July 10
Today we came up with a schematic of a possible decoder:
We looked more closely at the biobricks: BBa_I14032, BBa_R0040, BBa_K137125, BBa_K091101.
BBa_R0040 and BBa_K091101 were available to us in the registry distribution so we transformed these into cells to test them.
July 13
Concerns: We want the sRNA gene on a high copy plasmid and the target sequence on low copy plasmid. This could require up to 7 different plasmids to transform into the cell. We need to look into the feasibility of this.
