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| | Today we performed a midiprep to extract the plasmid DNA from dH5α. | | Today we performed a midiprep to extract the plasmid DNA from dH5α. |
| | + | |
| | + | '''Hybrid Promoter''' |
| | + | |
| | + | Today we came up with a schematic of a possible decoder: [[Image:IllinoisDecoderjuly10.png|200px|thumb|center]] |
| | + | |
| | + | |
| | + | We looked more closely at the biobricks: BBa_I14032, BBa_R0040, BBa_K137125, BBa_K091101. |
| | + | |
| | + | BBa_R0040 and BBa_K091101 were available to us in the registry distribution so we transformed these into cells to test them. |
| | | | |
| | | | |
| | [https://2009.igem.org/Team:Illinois/Notebook Back to Notebook page] | | [https://2009.igem.org/Team:Illinois/Notebook Back to Notebook page] |
| June
|
| M | T | W | T | F | S | S
|
| [http://2009.igem.org/Illinois/1_June_2009 1]
| [http://2009.igem.org/wiki/index.php?title=Illinois/2_June_2009&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Illinois/3_June_2009&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Illinois/4_June_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Illinois/5_June_2009&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Illinois/6_June_2009&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Illinois/7_June_2009&action=edit 7]
|
| [http://2009.igem.org/wiki/index.php?title=Illinois/8_June_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Illinois/9_June_2009&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Illinois/10_June_2009&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Illinois/11_June_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Illinois/12_June_2009&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Illinois/13_June_2009&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Illinois/14_June_2009&action=edit 14]
|
| [http://2009.igem.org/Illinois/15_June_2009 15]
| [http://2009.igem.org/Illinois/16_June_2009 16]
| [http://2009.igem.org/Illinois/17_June_2009 17]
| [http://2009.igem.org/Illinois/18_June_2009 18]
| [http://2009.igem.org/Illinois/19_June_2009 19]
| [http://2009.igem.org/wiki/index.php?title=Illinois/20_June_2009&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Illinois/21_June_2009&action=edit 21]
|
| [http://2009.igem.org/wiki/index.php?title=Illinois/22_June_2009&action=edit 22]
| [http://2009.igem.org/Illinois/23_June_2009 23]
| [http://2009.igem.org/Illinois/24_June_2009 24]
| [http://2009.igem.org/Illinois/25_June_2009 25]
| [http://2009.igem.org/Illinois/26_June_2009 26]
| [http://2009.igem.org/wiki/index.php?title=Illinois/27_June_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Illinois/28_June_2009&action=edit 28]
|
| [http://2009.igem.org/Illinois/29_June_2009 29]
| [http://2009.igem.org/Illinois/30_June_2009 30]
|
|
| July
|
| M | T | W | T | F | S | S
|
|
|
| [http://2009.igem.org/Illinois/1_July_2009 1]
| [http://2009.igem.org/Illinois/2_July_2009 2]
| [http://2009.igem.org/Illinois/3_July_2009 3]
| [http://2009.igem.org/wiki/index.php?title=Illinois/4_July_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Illinois/5_July_2009&action=edit 5]
|
| [http://2009.igem.org/Illinois/6_July_2009 6]
| [http://2009.igem.org/Illinois/7_July_2009 7]
| [http://2009.igem.org/wiki/index.php?title=Illinois/8_July_2009&action=edit 8]
| [http://2009.igem.org/Illinois/9_July_2009 9]
| [http://2009.igem.org/Illinois/10_July_2009 10]
| [http://2009.igem.org/wiki/index.php?title=Illinois/11_July_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Illinois/12_July_2009&action=edit 12]
|
| [http://2009.igem.org/Illinois/13_July_2009 13]
| [http://2009.igem.org/Illinois/14_July_2009 14]
| [http://2009.igem.org/Illinois/15_July_2009 15]
| [http://2009.igem.org/Illinois/16_July_2009 16]
| [http://2009.igem.org/Illinois/17_July_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Illinois/18_July_2009&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Illinois/19_July_2009&action=edit 19]
|
| [http://2009.igem.org/Illinois/20_July_2009 20]
| [http://2009.igem.org/Illinois/21_July_2009 21]
| [http://2009.igem.org/Illinois/22_July_2009 22]
| [http://2009.igem.org/Illinois/23_July_2009 23]
| [http://2009.igem.org/Illinois/24_July_2009 24]
| [http://2009.igem.org/wiki/index.php?title=Illinois/25_July_2009&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Illinois/26_July_2009&action=edit 26]
|
| [http://2009.igem.org/wiki/index.php?title=Illinois/27_July_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Illinois/28_July_2009&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Illinois/29_July_2009&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Illinois/30_July_2009&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Illinois/31_July_2009&action=edit 31]
|
|
| August
|
| M | T | W | T | F | S | S
|
|
|
|
|
|
| [http://2009.igem.org/wiki/index.php?title=Illinois/1_August_2009&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Illinois/2_August_2009&action=edit 2]
|
| [http://2009.igem.org/wiki/index.php?title=Illinois/3_August_2009&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Illinois/4_August_2009&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Illinois/5_August_2009&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Illinois/6_August_2009&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Illinois/7_August_2009&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Illinois/8_August_2009&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Illinois/9_August_2009&action=edit 9]
|
| [http://2009.igem.org/wiki/index.php?title=Illinois/10_August_2009&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Illinois/11_August_2009&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Illinois/12_August_2009&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Illinois/13_August_2009&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Illinois/14_August_2009&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Illinois/15_August_2009&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Illinois/16_August_2009&action=edit 16]
|
| [http://2009.igem.org/wiki/index.php?title=Illinois/17_August_2009&action=edit 17]
| [http://2009.igem.org/wiki/index.php?title=Illinois/18_August_2009&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Illinois/19_August_2009&action=edit 19]
| [http://2009.igem.org/wiki/index.php?title=Illinois/20_August_2009&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Illinois/21_August_2009&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Illinois/22_August_2009&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Illinois/23_August_2009&action=edit 23]
|
| [http://2009.igem.org/wiki/index.php?title=Illinois/24_August_2009&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Illinois/25_August_2009&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Illinois/26_August_2009&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Illinois/27_August_2009&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Illinois/28_August_2009&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Illinois/29_August_2009&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Illinois/30_August_2009&action=edit 30]
|
| [http://2009.igem.org/wiki/index.php?title=Illinois/31_August_2009&action=edit 31]
|
|
July 10
sRNA Library
Hopefully now the ligation will be working. After many many attempts we got a decent looking gel! The resolution isnt very good. This was an attempt to fit both the insert and the vector on the same gel but that didn't work at all. Its 1% with a 1kbp ladder.
The large, slow bands are hopefully the ligated plasmids. The ones just below that don't look like they worked. They look reasonably bright. Each lane is a variant of Insert:Vector ratio, ranging from 3:1 to 15:1 which were all ligated at room temperature for 4 hrs. (Protocol called for 1hr)
3'-Acting sRNAs
Today we performed a midiprep to extract the plasmid DNA from dH5α.
Hybrid Promoter
Today we came up with a schematic of a possible decoder:
We looked more closely at the biobricks: BBa_I14032, BBa_R0040, BBa_K137125, BBa_K091101.
BBa_R0040 and BBa_K091101 were available to us in the registry distribution so we transformed these into cells to test them.
Back to Notebook page
"
