Team:Wash U/Calendar-Home/14 July 2009

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(Difference between revisions)
(→Tuesday, July 14, 2009)
(→Tuesday, July 14, 2009)
Line 14: Line 14:
1) Ladder
1) Ladder
-
2) #1 OmpC (108 bp)
+
2) OmpC (108 bp)
-
3) #1 OmpC
+
3) OmpC
-
4) #1 GFP (876 bp)
+
4) GFP (876 bp)
-
5) #1 GFP
+
5) GFP
6) Ladder
6) Ladder
Line 28: Line 28:
1) Ladder
1) Ladder
-
2) #2 OmpC
+
2) OmpC
-
3) #2 OmpC
+
3) OmpC
-
4) #2 Destination plasmid (pSB1K3)
+
4) Destination plasmid (pSB1K3)
-
5) #2 Dest. plasmid (pSB1K3)
+
5) Dest. plasmid (pSB1K3)
-
6) #2 GFP
+
6) GFP
-
7) #2 GFP
+
7) GFP
8) Ladder
8) Ladder

Revision as of 21:33, 14 July 2009

Tuesday, July 14, 2009

-Colony PCR of cells transformed with previous ligations
-PCR to add KpnI and BamHI sites to GFP
-Spectrophotometry of dbcOmega strains in ATCC media
-PCR to add BioBrick prefix and suffix to Cph8 and pRKCBC3
-Transformed S17 competent cells to check efficiency. OD = 0.115
-Performed Triligation and Silver Lab Ligations to fuse OmpC+GFP
-Started cultures of dbcOmega in LB+Strep in lieu of ATCC

Gel #1: Silver digestion protocol

1) Ladder

2) OmpC (108 bp)

3) OmpC

4) GFP (876 bp)

5) GFP

6) Ladder

Gel #2: BioBrick (Triligation) digestion protocol

1) Ladder

2) OmpC

3) OmpC

4) Destination plasmid (pSB1K3)

5) Dest. plasmid (pSB1K3)

6) GFP

7) GFP

8) Ladder


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