Team:Wash U/Protocol
From 2009.igem.org
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#Dilute bilin mixture 10-fold with 0.1% (v/v) TFA. | #Dilute bilin mixture 10-fold with 0.1% (v/v) TFA. | ||
#Apply to C18 Sep-Pak cartridge. | #Apply to C18 Sep-Pak cartridge. | ||
- | #Wash cartridge with 0.1% (v/v)TFA (2x 3ml) and acetonitrile/0.1% TFA (20:80; 2x 2ml. | + | #Wash cartridge with 0.1% (v/v)TFA (2x 3ml) and acetonitrile/0.1% TFA (20:80; 2x 2ml). |
#Elute bilin with 3ml acetonitriel/0.1% TFA (60:40) and dry in vacuo. | #Elute bilin with 3ml acetonitriel/0.1% TFA (60:40) and dry in vacuo. | ||
:Note:Expected yields are 4 umol PCB per 6g Spirulina | :Note:Expected yields are 4 umol PCB per 6g Spirulina |
Revision as of 20:07, 15 July 2009