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Virginia Commonwealth/16 July 2009
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(New page: ==Saturday 06 June 2009== ===Results=== * Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation {| cellpadding="10" align="center" border="0.9" padd...)
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(New page: ==Saturday 06 June 2009== ===Results=== * Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation {| cellpadding="10" align="center" border="0.9" padd...)
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Revision as of 14:31, 16 July 2009
Contents |
Saturday 06 June 2009
Results
- Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation
| Promoter | Reporter | Resistance/Plate | Growth Observation | |
|---|---|---|---|---|
| J23101 | E0240 | Cm | no growth | |
| J23101 | J06702 | Cm | 21 colonies | |
| J23102 | E0240 | Cm | 3 colonies | |
| J23102 | J06702 | Cm | 8 colonies | |
| J23103 | E0240 | Cm | 2 colonies | |
| J23103 | J06702 | Cm | 23 colonies | |
| J23104 | E0240 | Cm | 9 colonies | |
| J23104 | J06702 | Cm | 12 colonies | |
| J23105 | E0240 | Cm | no growth | |
| J23105 | J06702 | Cm | 14 colonies | |
| J23107 | E0240 | Cm | 3 colonies | |
| J23107 | J06702 | Cm | 3 colonies |
- No growth was seen in 5mL culture taken from NEB 10 beta cell freezer stock
- Vial may have thawed out too much after cells were taken out for the electrocompetency procedure
Trentay 14:31, 16 July 2009 (UTC)
Tasks
Maria and Afton
- Digest: E0240, pSB4C5, 110, 113, 116,
Wetlab
Maria and Afton
