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Virginia Commonwealth/15 July 2009
From 2009.igem.org
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* Organize Notebook(s) | * Organize Notebook(s) | ||
[[User:Trentay|Trentay]] 16:59, 15 July 2009 (UTC) | [[User:Trentay|Trentay]] 16:59, 15 July 2009 (UTC) | ||
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| + | ''Craig and Clay'' | ||
| + | * The cells growing on the non-selective media will be streaked onto plates containing kanamycin and plates containing chloramphenicol. | ||
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* 10% glycerol stock was made | * 10% glycerol stock was made | ||
[[User:Trentay|Trentay]] 14:13, 16 July 2009 (UTC) | [[User:Trentay|Trentay]] 14:13, 16 July 2009 (UTC) | ||
| + | |||
| + | ''Craig and Clay'' | ||
| + | * Cells possibly containing the limonene synthesis related parts grown on the plates containing no antibiotics were taken and plated onto kanamycin-selective and chloramphenicol-selective plates. | ||
Revision as of 18:08, 17 July 2009
Contents |
Saturday 06 June 2009
Results
- Results of Retransformation with pSB4C5 backbone in NEB 10 beta
| Promoter | Reporter | Resistance/Plate | Growth Observation | |
|---|---|---|---|---|
| J23101 | E0240 | Cm | ||
| J23101 | J06702 | Cm | ||
| J23102 | E0240 | Cm | ||
| J23102 | J06702 | Cm | ||
| J23103 | E0240 | Cm | ||
| J23103 | J06702 | Cm | ||
| J23104 | E0240 | Cm | ||
| J23104 | J06702 | Cm | ||
| J23105 | E0240 | Cm | ||
| J23105 | J06702 | Cm | ||
| J23107 | E0240 | Cm | ||
| J23107 | J06702 | Cm |
- Controls with NEB 10 beta
| Control | Resistance | Plate | Growth Observation | |
|---|---|---|---|---|
| Positive (+) | none | LB | lawn | |
| Electroshocked (+) | none | LB | lawn | |
| Negative (-) | none | Cm | no cell growth |
Trentay 16:59, 15 July 2009 (UTC)
Craig and Clay
- The ampicillin plates again showed no growth. However, the non-selective plates displayed lawn growth.
Tasks
Maria and Afton
- Make more electrocompetent NEB 10 beta
- 500 mL LB autoclaved in 1 L flask
- 1 L LB autoclaved
- 2mL of NEB 10 beta overnight culture was diluted in 4mL of LB broth
- Two bottles of LB Agar were prepared
- for Amp and Cm plates
- Three bottles of LB prepared
- two liquid LB+Amp will be prepared
- one liquid LB+Cm will be prepared
- Let transformed BioBrick parts continue to grow overnight (on plates) before colonies are picked
- Make cell stock of Biobrick part: J23106, J06702, pSB4C5
- 2mL of BioBrick overnight culture was diluted in 4mL of LB broth
- Organize Notebook(s)
Trentay 16:59, 15 July 2009 (UTC)
Craig and Clay
- The cells growing on the non-selective media will be streaked onto plates containing kanamycin and plates containing chloramphenicol.
Wetlab
Maria and Afton
- All LB was prepared and autoclaved
- Overnight culture dilutions were completed
- Electrocompetency of NEB 10 beta was completed
- LB and Sterile water was made
- 10% glycerol stock was made
Trentay 14:13, 16 July 2009 (UTC)
Craig and Clay
- Cells possibly containing the limonene synthesis related parts grown on the plates containing no antibiotics were taken and plated onto kanamycin-selective and chloramphenicol-selective plates.
