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Indiana/6 July 2009
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(New page: Grow liquid culture to prepare for mini prep Preparation of Media: The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made...)
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(New page: Grow liquid culture to prepare for mini prep Preparation of Media: The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made...)
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Revision as of 18:59, 19 July 2009
Grow liquid culture to prepare for mini prep
Preparation of Media:
The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made.
Kan media: 50 mL LB 50 uL KAN
Amp media: 50 mL LB 50 uL amp
add 3 mL of media to a tube transfer 1 colony into each tube shake @ 37 C o/n
