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Indiana/6 July 2009
From 2009.igem.org
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| - | Grow liquid culture to prepare for mini prep | + | '''Grow liquid culture to prepare for mini prep''' |
| - | + | Preparation of Media: | |
The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made. | The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made. | ||
Revision as of 19:06, 19 July 2009
Grow liquid culture to prepare for mini prep
Preparation of Media:
The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made.
Kan media:
50 mL LB
50 uL KAN
Amp media:
50 mL LB
50 uL amp
add 3 mL of media to a tube
transfer 1 colony into each tube
shake @ 37 C o/n
