Team:SDU-Denmark/Project

From 2009.igem.org

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<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:SDU-Denmark|Home]]
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!align="center"|[[Team:SDU-Denmark/Team|The Team]]
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!align="center"|[[Team:SDU-Denmark/Project|The Project]]
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!align="center"|[[Team:SDU-Denmark/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:SDU-Denmark/Modeling|Modeling]]
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!align="center"|[[Team:SDU-Denmark/Notebook|Notebook]]
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== '''Overall project''' ==
 
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=== Bactobandage ===
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[[Team:SDU-Denmark|Home]] | [[Team:SDU-Denmark/Background|Background]] | [[Team:SDU-Denmark/Project|Project]] | [[Team:SDU-Denmark/Parts|Parts]] | [[Team:SDU-Denmark/Team|Team]]
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Inhibition of quorum-sensing mediated biofilm-formation in staphylococcus aureus.
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[[Team:SDU-Denmark/Diary|Diary]] | [[Team:SDU-Denmark/Protocols|Protocols]] | [[Team:SDU-Denmark/Downloads|Downloads]] | [[Team:SDU-Denmark/Brainstorm|Brainstorm]]
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We're going to stop Staphylococcus aureus infections (not colonization) by inhibiting the quorum sensing-dependent biofilm formation
 
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[[Image:SDU-Denmark-Staphylococcus_aureus%2C_50%2C000x%2C_USDA%2C_ARS%2C_EMU.jpg|200px|thumb|right| Staphyloccocus Aureus]]
 
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=='''Initial phase planning'''==
 
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=== Preliminary Project Details===
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'''Brainstorm:'''
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The details we need to settle on soon (eg. by Thursday) are:
 
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1) Which bacteria is the producer in the bacto bandage (suggestions: E. coli, Lactococcus, ??)
 
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2) Identifying the natural producer of the quorum quenching signal (QQS)
 
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3) Which proteins should be expressed
 
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4) How should expression be regulated
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'''Forslag til opbygning:'''
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5) How do we retain plasmid stability in the bactobandage
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1. Concept
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6) More?
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2. Metode and results
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3. Summary of results
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=== Initial Experimental Considerations ===
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4. Implications and development
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Before we can get started a number of experimental procedures need to be discussed and/or protocols need to be identified:
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Initial lab work:
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1) Making competent E. Coli for our freezer
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2) Amplification of basic parts in E. Coli
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3) Plasmid purification (maxi prep og mini prep)
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4) Growth and handling of reciever bacteria
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5) Identify and amplify our favourite plasmid backbones
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Biobrick expression:
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1) Planning and ordering primers
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2) Amplification of target gene from chromosomal DNA (PCR) 
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3) Manipulation of biobrick part and composite parts
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4) Transformation/electroporation into producer bacteria
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Testing output:
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1) Western blot (protein)
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2) Northern blot (RNA)
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3) Promoter LaxZ or XFP fusions
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4) RT-PCR
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5) Sequencing (a service we buy)
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6) More???
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== Experiments ==
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== Results ==
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Revision as of 21:55, 23 July 2009


Home | Background | Project | Parts | Team

Diary | Protocols | Downloads | Brainstorm


Brainstorm:



Forslag til opbygning:

1. Concept

2. Metode and results

3. Summary of results

4. Implications and development

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