Virginia Commonwealth/16 July 2009

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(Results)
(Tasks)
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* Transformation of NEB 10 beta to make sure new stock is electrocompetent
* Transformation of NEB 10 beta to make sure new stock is electrocompetent
[[User:Trentay|Trentay]] 20:41, 16 July 2009 (UTC)
[[User:Trentay|Trentay]] 20:41, 16 July 2009 (UTC)
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''Craig and Clay''
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* The registry will be contacted to verify the correct resistence for the limonene synthesis related parts.  New DNA will also be requested.
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Revision as of 14:15, 24 July 2009

Contents

Thursday 16 July 2009

Results

  • Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation
Promoter Reporter Resistance/Plate Growth Observation
J23101 E0240 Cm no growth
J23101 J06702 Cm 21 colonies
J23102 E0240 Cm 3 colonies
J23102 J06702 Cm 8 colonies
J23103 E0240 Cm 2 colonies
J23103 J06702 Cm 23 colonies
J23104 E0240 Cm 9 colonies
J23104 J06702 Cm 12 colonies
J23105 E0240 Cm no growth
J23105 J06702 Cm 14 colonies
J23107 E0240 Cm 3 colonies
J23107 J06702 Cm 3 colonies
  • No growth was seen in 5mL culture taken from NEB 10 beta cell freezer stock
    • Vial may have thawed out too much after cells were taken out for the electrocompetency procedure

Trentay 14:31, 16 July 2009 (UTC)

--Craig and Clay--

  • No growth was seen on either the kanamycin or chloramphenicol plates.
  • The sequence for the LIM1 gene was verified to be optimized for E. coli.

Tasks

Maria and Afton

  • Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
  • Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
  • Run an electrophoresis test on promoters and reporter Eo240
  • Pick colonies of promoters from transformation 100-107 (excluding 106)
  • Transformation of NEB 10 beta to make sure new stock is electrocompetent

Trentay 20:41, 16 July 2009 (UTC)

Craig and Clay

  • The registry will be contacted to verify the correct resistence for the limonene synthesis related parts. New DNA will also be requested.

Wetlab

Maria and Afton

  • Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
  • Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
  • Run an electrophoresis test on promoters and reporter Eo240

Trentay 20:41, 16 July 2009 (UTC)