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Team:Groningen/Parts
From 2009.igem.org
(Difference between revisions)
m (→Part List -80) |
(→Part List -80) |
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|Ampicillin/Gentamycin | |Ampicillin/Gentamycin | ||
|plate 3, pos.2 | |plate 3, pos.2 | ||
| + | |- | ||
| + | |(?)pHigh in low copy nr vector | ||
| + | |High consitutive promoter | ||
| + | |<partinfo>J23100</partinfo> | ||
| + | |35bp | ||
| + | |<partinfo>pSB3K3</partinfo> | ||
| + | |Kanamycin | ||
| + | |Plate 3, pos. 4 | ||
| + | |- | ||
| + | |(?)pMed in low copy nr vector | ||
| + | |Medium consitutive promoter | ||
| + | |<partinfo>J23106</partinfo> | ||
| + | |35bp | ||
| + | |<partinfo>pSB3K3</partinfo> | ||
| + | |Kanamycin | ||
| + | |Plate 3, pos. 5 | ||
| + | |- | ||
| + | |(?)pLow in low copy nr vector | ||
| + | |Low consitutive promoter | ||
| + | |<partinfo>J23109</partinfo> | ||
| + | |35bp | ||
| + | |<partinfo>pSB3K3</partinfo> | ||
| + | |Kanamycin | ||
| + | |Plate 3, pos. 6 | ||
| + | |- | ||
| + | |(?)pHigh in high copy nr vector | ||
| + | |High consitutive promoter | ||
| + | |<partinfo>J23100</partinfo> | ||
| + | |35bp | ||
| + | |<partinfo>pSB1AC3</partinfo> | ||
| + | |Kanamycin | ||
| + | |Plate 3, pos. 7 | ||
| + | |- | ||
| + | |(?)pMed in high copy nr vector | ||
| + | |Medium consitutive promoter | ||
| + | |<partinfo>J23106</partinfo> | ||
| + | |35bp | ||
| + | |<partinfo>pSB1AC3</partinfo> | ||
| + | |Kanamycin | ||
| + | |Plate 3, pos. 8 | ||
| + | |- | ||
| + | |(?)pLow in high copy nr vector | ||
| + | |High consitutive promoter | ||
| + | |<partinfo>J23109</partinfo> | ||
| + | |35bp | ||
| + | |<partinfo>pSB1AC3</partinfo> | ||
| + | |Kanamycin | ||
| + | |Plate 3, pos. 9 | ||
|- | |- | ||
|Promotor (low expression) | |Promotor (low expression) | ||
| Line 64: | Line 112: | ||
|Ampicillin | |Ampicillin | ||
|plate 3, pos.12 | |plate 3, pos.12 | ||
| + | |- | ||
| + | |Inducible promoter | ||
| + | |Lactose inducible promoter nr1 | ||
| + | |<partinfo>R0010</partinfo> | ||
| + | |200bp | ||
| + | |<partinfo>pSB1A2</partinfo> | ||
| + | |Ampicillin | ||
| + | |Plate 3, pos. 14 | ||
| + | |- | ||
| + | |Inducible promoter | ||
| + | |Lactose inducible promoter nr2 | ||
| + | |<partinfo>R0010</partinfo> | ||
| + | |200bp | ||
| + | |<partinfo>pSB1A2</partinfo> | ||
| + | |Ampicillin | ||
| + | |Plate 3, pos. 15 | ||
|- | |- | ||
|RBS | |RBS | ||
Revision as of 12:18, 25 July 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
| Biobricks
|
|---|
Note
If you choose to include a Parts Submitted to the Registry page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
- http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen Our sandbox]
Part List -80
| Component | Description | Part or Accession # | Base Pairs (bp) | Plasmid (backbone) | Resistance | Well |
| GVP | Gas Vesicle Proteins | [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] | 6064 | [http://partsregistry.org/Part:BBa_J61035 BBa_J61035] | Ampicillin/Gentamycin | plate 3, pos.1 |
| GVP (2nd batch) | Gas Vesicle Proteins | [http://partsregistry.org/Part:BBa_I750016 BBa_I750016] | 6064 | [http://partsregistry.org/Part:BBa_J61035 BBa_J61035] | Ampicillin/Gentamycin | plate 3, pos.2 |
| (?)pHigh in low copy nr vector | High consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 4 | ||
| (?)pMed in low copy nr vector | Medium consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 5 | ||
| (?)pLow in low copy nr vector | Low consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 6 | ||
| (?)pHigh in high copy nr vector | High consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 7 | ||
| (?)pMed in high copy nr vector | Medium consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 8 | ||
| (?)pLow in high copy nr vector | High consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 9 | ||
| Promotor (low expression) | Constitutive promotor | [http://partsregistry.org/Part:BBa_J23109 BBa_J23109] | 35 | [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] | Ampicillin | plate 3, pos.10 |
| Promotor (medium expression) | Constitutive promotor | [http://partsregistry.org/Part:BBa_J23106 BBa_J23106] | 35 | [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] | Ampicillin | plate 3, pos.11 |
| Promotor (high expression) | Constitutive promotor | [http://partsregistry.org/Part:BBa_J23100 BBa_J23100] | 35 | [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] | Ampicillin | plate 3, pos.12 |
| Inducible promoter | Lactose inducible promoter nr1 | 200bp | Ampicillin | Plate 3, pos. 14 | ||
| Inducible promoter | Lactose inducible promoter nr2 | 200bp | Ampicillin | Plate 3, pos. 15 | ||
| RBS | Ribosomal Binding Site | [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] | 12 | [http://partsregistry.org/Part:pSB1A2 pSB1A2] | Ampicillin | plate 3, pos.19 |
| Terminator | Double terminator consisting of [http://partsregistry.org/Part:BBa_B0012 BBa_B0012] and [http://partsregistry.org/Part:BBa_B0011 BBa_B0011] | [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] | ~90 | [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] | Ampicillin/Kanamycin | plate 3, pos.20 |
| HmtA (1.1) | ? | None | ? | None | Ampicillin | plate 3, pos.28 |
| HmtA (2.1) | ? | None | ? | None | Ampicillin | plate 3, pos.29 |
