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EPF-Lausanne/17 July 2009
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(New page: ===Wet Lab=== The plates and tubes from yesterday transformation weren't very concluant : almost no colony on the plates (actually we saw 3 of them but this might be contamination) and for...)
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Wet Lab
The plates and tubes from yesterday transformation weren't very concluant : almost no colony on the plates (actually we saw 3 of them but this might be contamination) and for the tubes there were almost no bacteria. We decided to do the miniprep with it anyways, to see what it gives :
Miniprep of LovTAP-Term, CT negative of the ligation and Term (that grew very well).
PCR of the miniprep products.
Gel electrophoresis of the PCR products and also of the plasmid of LovTAP-Term, CT neg and Term. We can see on the gel that there was actually no DNA on the LovTAP-Term and CT negative tubes.
In parallel, we began all over again, by taking Term from the kit. We used a different method, as the other one seems not to be working (the Term plasmid seems not to accept LovTAP in it). So this time we used the iGEM protocol to do the digestion. As the P site is in LovTAP, which is the upstream part, it is not a problem.
We did the digestion, ligation and transformation of LovTAP-Term and this time we really hope this will work.
People in the lab
- Heidi, Tu, Nath, Caro, Nicolas
