"
Team:MoWestern Davidson/project design
From 2009.igem.org
(New page: {{Template:MoWestern_Davidson2009}} Project Design / Approaches Continuum (scale) FSL Design/tRNAS To solve the SAT problem, we constructed reporter genes that express a phenotype in t...)
Newer edit →
Revision as of 16:29, 27 July 2009
Project Design / Approaches Continuum (scale) FSL Design/tRNAS To solve the SAT problem, we constructed reporter genes that express a phenotype in the event E. coli solves the SAT problem. To design these reporter genes, we constructed a continuum to model the different level of bacterial automation in solving the SAT problem. This enabled us to select the level of automation we desired and design reporter genes based off of that level. The basic concept behind the reporter genes involves inserting a 5 base pair insertion. This insertion serves to throw off the reading frame in translation, causing a nonsense protein. We then provide the cells with different suppressor tRNAs that may or may not suppress this insertion. If the insertion is suppressed, the reading frame will be restored and the protein will be expressed. These suppressors represent the inputs of the SAT problem, and the 5 base pair insertions represent the clauses of the problem.
