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Team:Virginia/27 July 2009
From 2009.igem.org
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**2 cycles with 58C annealing temp, then 28 cycles with 65C annealing temp | **2 cycles with 58C annealing temp, then 28 cycles with 65C annealing temp | ||
**74C extension step, 10 sec | **74C extension step, 10 sec | ||
| + | **[[Image:Vgem072709.jpg]] | ||
| + | **Ladder, No template DNA, Template DNA & 1mM MgCl2,...& 2mM MgCl2,...& 4mM MgCl2 | ||
Latest revision as of 19:11, 27 July 2009
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Overnight Results
- All platings blank again
Today
- PCR to add HA tag to ABP
- 1ul of ABP DNA @ 422ng/ul
- 3 different MgCl2 concentrations: 1, 2, 4 mM
- Control w/o ABP DNA
- 2 cycles with 58C annealing temp, then 28 cycles with 65C annealing temp
- 74C extension step, 10 sec
- Ladder, No template DNA, Template DNA & 1mM MgCl2,...& 2mM MgCl2,...& 4mM MgCl2
