EPF-Lausanne/7 July 2009
From 2009.igem.org
(Difference between revisions)
(→People in the lab) |
(→People in the lab) |
||
Line 54: | Line 54: | ||
:Tu, Heidi, Rafael, Basile, Nath | :Tu, Heidi, Rafael, Basile, Nath | ||
- | <html><center><a href="https://2009.igem.org/EPF-Lausanne/6_July_2009"><img src="https://static.igem.org/mediawiki/2009/6/61/Fl%C3%A8che_gauche.png/70px- | + | [[Image:Flèche_gauche.png | gauache]] |
+ | |||
+ | <!- | ||
+ | <html><center><a href="https://2009.igem.org/EPF-Lausanne/6_July_2009"><img src="https://static.igem.org/mediawiki/2009/6/61/Fl%C3%A8che_gauche.png/70px-Fl%C3%A8che_gauche.png"></a></center></html> ---> | ||
<html><center><a href="https://2009.igem.org/EPF-Lausanne/7_July_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/5/5e/Fleche_droite.png/70px-Fleche_droite.png"></a></center></html> | <html><center><a href="https://2009.igem.org/EPF-Lausanne/7_July_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/5/5e/Fleche_droite.png/70px-Fleche_droite.png"></a></center></html> | ||
Revision as of 08:46, 28 July 2009
Wet Lab
We have to grow the 3 strains generously sent by Tom Beatty
The three strains are :
- R.Palustris CEA001 (wild type) ; should be grown on LB medium only
- R.Palustris BPHP1+ ; should be grown on LB with gentamycin (100 micrograms/ml)
- E.Coli DH10B (pBPH/hmu0) ; should be grown on LB with gentamycin (20 micorgrams/ml)
The transformed LOVTAP and TrpR worked well (N.B. the plasmid of TrpR is pUC19 so the antibiotic resistance is Amp -> see below)
We did the glycerol stock, located in the -80 fridge, first floor of the iGEM compartement.
Then, a miniprep was done with both cultures. A LOVTAP plasmid aliquot was done, a TrpR plasmid aliquot was done, located in the -20 fridge, 2nd floor.
Cloning Strategy
To design plasmids : software Vector NTI
People in the lab
- Tu, Heidi, Rafael, Basile, Nath
<!-