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Uppsala-Sweden/27 July 2009
From 2009.igem.org
(Difference between revisions)
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2009-07-27 11:15,OD measurement of Butanol at 750nm, | 2009-07-27 11:15,OD measurement of Butanol at 750nm, | ||
| - | pirA PCR, | + | pirA PCR was conducted, using |
1. Primer:pirA forward | 1. Primer:pirA forward | ||
pirA reverse | pirA reverse | ||
pirB forward | pirB forward | ||
| - | at different template concentration, | + | at three different template concentration, |
2. Primer:pirA reverse | 2. Primer:pirA reverse | ||
pirB forward | pirB forward | ||
| + | at three different template concentration, | ||
Control pirB | Control pirB | ||
| - | + | ||
| + | PCR PROGRAM | ||
| + | denaturing temperature 98 | ||
| + | denaturing time: 30s | ||
| + | cycle: 5 | ||
| + | denaturing temperature 98 | ||
| + | denaturing time: 10s | ||
| + | anealing temperature: 63, time 30s | ||
| + | extension time:32s | ||
| + | extension temperature 72 | ||
| + | cycle: 30 | ||
| + | denaturing temperature 98 | ||
| + | denaturing time: 10s | ||
| + | annealing temperature: 72,time 30s | ||
| + | extension time:32s | ||
| + | extension temperature 72 | ||
| + | hold 5 min | ||
| + | |||
| + | |||
| + | |||
-- Emily | -- Emily | ||
Latest revision as of 14:10, 30 July 2009
2009-07-27 11:15,OD measurement of Butanol at 750nm,
pirA PCR was conducted, using 1. Primer:pirA forward
pirA reverse
pirB forward
at three different template concentration,
2. Primer:pirA reverse
pirB forward at three different template concentration,
Control pirB
PCR PROGRAM denaturing temperature 98 denaturing time: 30s cycle: 5 denaturing temperature 98 denaturing time: 10s anealing temperature: 63, time 30s extension time:32s extension temperature 72 cycle: 30 denaturing temperature 98 denaturing time: 10s annealing temperature: 72,time 30s extension time:32s extension temperature 72 hold 5 min
-- Emily
