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Team:Todai-Tokyo/Notebook/bioclock
From 2009.igem.org
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Miniprep again of E.coli cells containing LDLR gene with Promega, Wizard Plus SV Miniprep DNA Purification System<BR> | Miniprep again of E.coli cells containing LDLR gene with Promega, Wizard Plus SV Miniprep DNA Purification System<BR> | ||
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Miniprep(7/29)<BR> | Miniprep(7/29)<BR> | ||
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#P1.9C(p15A) | #P1.9C(p15A) | ||
#P1.9G(p15A) | #P1.9G(p15A) | ||
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| + | 7/30 | ||
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| + | Miniprep | ||
| + | #P1.14L(araC) | ||
| + | #P1.7L(lacI) | ||
<!--- The Mission, Experiments ---> | <!--- The Mission, Experiments ---> | ||
Revision as of 15:14, 30 July 2009
Plan
Aim: Create E.coli that show oscillatory gene expression pattern
Methods:
1. Clone the following genes from Bacillus subtilis into a biobrick vector.
- plate1,13B
- plate1,13L
- plate2,1H
2. Make a gene network that express oscillatory pattern, using these genes.
Cloning the parts
7/7
preculture of the Biobrick parts for Miniprep
7/8
Miniprep of E.coli cells containing LDLR gene with Promega, Wizard Plus SV Miniprep DNA Purification System
failure
7/9
Miniprep again of E.coli cells containing LDLR gene with Promega, Wizard Plus SV Miniprep DNA Purification System
Miniprep(7/29)
- P1.14L(araC)
- P1.7L(lacI)
- P1.4E(cI)
- P1.3D(ColE1)
- P1.9C(p15A)
- P1.9G(p15A)
7/30
Miniprep
- P1.14L(araC)
- P1.7L(lacI)
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