Revision as of 11:10, 31 July 2009

Used Parts
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=KULeuven Submitted Parts]

Blue light receptor

part	plasmid	description	status	μl left	concentration	date
MC4100	/	strain of E.coli				july 24
2171	/	forward primer for K23800		90µl	20μM	july 24
2172	/	reverse primer for K23800		90µl	20μM	july 24
2171	/	forward primer for K238000			100μM	july 24
2172	/	reverse primer for K238000			100μM	july 24
E0240	pSB1A2	GFP + rbs + terminator	purified			july 29
E0240	pSB1A2	GFP + rbs + terminator	restricted with EcoRI and XbaI			july 29
	/	BLR promoter region	restricted with EcoRI			july 29
K238000	/	BLR promoter region	4 microcentrifuge tubes			july 30

part	plasmid	description	status	μl left	concentration	date
	/	GFP with LVA tag	purified			july 28
	/	tetR promoter	purified			july 28

part	plasmid	description	status	μl left	concentration	date
	pSB1A2	ech + rbs	put in competent cells			july 28
	pSB1A2	fcs + rbs	put in competent cells			july 28

part	plasmid	description	status	μl left	concentration	date

part	plasmid	description	μl left	concentration	date
dNTP mix		ATP, GTP, TTP, CTP	94	10μM	July 24
	pSB1A2	medium constitutieve promoter			July 23
ampicilline

@@ Line 90: / Line 90: @@
 |-
 |ampicilline||||||||||||
-|}
-= Primers =
-{| class="Generic"
-! class='top' | Number
-! class='top' | Name
-! class='top' | Sequence
-! class='top' | Length
-! class='top' | Tm (°C)
-! class='top' | Comments
-|-
-|2171||BLR promoter fw||CATCAT GAATTCGCGGCCGCTTCTAGAG  TTT GAC AGG TTC GTC GTC||46||52.4||it has a catcat en prefix (not in Tm included)
-|-
-|2172||BLR promoter rv||CTGCAGCGGCCGCTACTAGTA   CCT CTG TTA AAA ATG TTA ATC AAT GTT AAG||51||52.1||it has a suffix (not in Tm included)
 |}