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Team:KULeuven/Freezer
From 2009.igem.org
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| - | | | + | | {{kulpart|BBa_MC4100}} || / || strain of E.coli || || || || july 24 |
|- | |- | ||
| - | | 2171 || / ||forward primer for | + | | 2171 || / ||forward primer for {{kulpart|BBa_K238000}} || || 90µl || 20μM ||july 24 |
|- | |- | ||
| - | | 2172 || / || reverse primer for | + | | 2172 || / || reverse primer for {{kulpart|BBa_K238000}} || || 90µl || 20μM || july 24 |
|- | |- | ||
| - | | 2171 || / || forward primer for | + | | 2171 || / || forward primer for {{kulpart|BBa_K238000}} || || || 100μM || july 24 |
|- | |- | ||
| - | | 2172 || / || reverse primer for | + | | 2172 || / || reverse primer for {{kulpart|BBa_K238000}} || || || 100μM || july 24 |
|- | |- | ||
| {{kulpart|BBa_E0240}}||pSB1A2||GFP + rbs + terminator||purified||||||july 29 | | {{kulpart|BBa_E0240}}||pSB1A2||GFP + rbs + terminator||purified||||||july 29 | ||
Revision as of 11:12, 31 July 2009
Contents |
Blue light receptor
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| / | strain of E.coli | july 24 | ||||
| 2171 | / | forward primer for | 90µl | 20μM | july 24 | |
| 2172 | / | reverse primer for | 90µl | 20μM | july 24 | |
| 2171 | / | forward primer for | 100μM | july 24 | ||
| 2172 | / | reverse primer for | 100μM | july 24 | ||
| pSB1A2 | GFP + rbs + terminator | purified | july 29 | |||
| pSB1A2 | GFP + rbs + terminator | restricted with EcoRI and XbaI | july 29 | |||
| / | BLR promoter region | restricted with EcoRI | july 29 | |||
| / | BLR promoter region | 4 microcentrifuge tubes | july 30 |
Key/antikey/lock
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| / | GFP with LVA tag | purified | july 28 | |||
| / | tetR promoter | purified | july 28 |
vanillin synthesis
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| pSB1A2 | ech + rbs | put in competent cells | july 28 | |||
| pSB1A2 | fcs + rbs | put in competent cells | july 28 |
vanillin receptor
| part | plasmid | description | status | μl left | concentration | date |
|---|
miscellaneous
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| dNTP mix | ATP, GTP, TTP, CTP | 94 | 10μM | July 24 | ||
| pSB1A2 | medium constitutieve promoter | July 23 | ||||
| ampicilline |
Categories: Team:KULeuven/BioBricks | Team:KULeuven/BioBricks/BBa MC4100 | Team:KULeuven/BioBricks/BBa K238000 | Team:KULeuven/BioBricks/BBa E0240 | Team:KULeuven/BioBricks/BBa K145015 | Team:KULeuven/BioBricks/BBa K145201 | Team:KULeuven/BioBricks/BBa I742113 | Team:KULeuven/BioBricks/BBa I742115 | Team:KULeuven/BioBricks/J23110
