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Team:KULeuven/Freezer
From 2009.igem.org
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| - | | | + | | MC4100 || / || strain of E.coli || || || || july 24 |
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| 2171 || / ||forward primer for {{kulpart|BBa_K238000}} || || 90µl || 20μM ||july 24 | | 2171 || / ||forward primer for {{kulpart|BBa_K238000}} || || 90µl || 20μM ||july 24 | ||
Revision as of 11:13, 31 July 2009
Contents |
Blue light receptor
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| MC4100 | / | strain of E.coli | july 24 | |||
| 2171 | / | forward primer for | 90µl | 20μM | july 24 | |
| 2172 | / | reverse primer for | 90µl | 20μM | july 24 | |
| 2171 | / | forward primer for | 100μM | july 24 | ||
| 2172 | / | reverse primer for | 100μM | july 24 | ||
| pSB1A2 | GFP + rbs + terminator | purified | july 29 | |||
| pSB1A2 | GFP + rbs + terminator | restricted with EcoRI and XbaI | july 29 | |||
| / | BLR promoter region | restricted with EcoRI | july 29 | |||
| / | BLR promoter region | 4 microcentrifuge tubes | july 30 |
Key/antikey/lock
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| / | GFP with LVA tag | purified | july 28 | |||
| / | tetR promoter | purified | july 28 |
vanillin synthesis
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| pSB1A2 | ech + rbs | put in competent cells | july 28 | |||
| pSB1A2 | fcs + rbs | put in competent cells | july 28 |
vanillin receptor
| part | plasmid | description | status | μl left | concentration | date |
|---|
miscellaneous
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| dNTP mix | ATP, GTP, TTP, CTP | 94 | 10μM | July 24 | ||
| pSB1A2 | medium constitutieve promoter | July 23 | ||||
| ampicilline |
