Virginia Commonwealth/31 July 2009

From 2009.igem.org

(Difference between revisions)
(New page: ===Results=== ''Maria and Afton'' * Overnight culture of I1352 grew, but no visible florescence * Transformation Results below {| cellpadding="10" align="center" border="0.9" padding="5" |...)
(→Results)
Line 1: Line 1:
 +
==Tuesday 7 July 2009==
 +
===Results===
===Results===
''Maria and Afton''
''Maria and Afton''
Line 24: Line 26:
|-
|-
! NEB 10 beta  
! NEB 10 beta  
-
| AMP || I1352 ligated to the backbone PSB4C5 || hundreds of colonies; some expressing RFP ||
+
| AMP || I1352 ligated to the backbone pSB4C5 || hundreds of colonies; some expressing RFP ||
|-
|-
! NEB 10 beta  
! NEB 10 beta  
Line 30: Line 32:
|}
|}
[[User:Trentay|Trentay]] 15:25, 31 July 2009 (UTC)
[[User:Trentay|Trentay]] 15:25, 31 July 2009 (UTC)
 +
* Interpretation of Transformation Results:
 +
** Shows the digestion/ligation process is not working
 +
** Because the I1352 grew successfully on an AMP plate after being 'ligated' with pSB4C5, shows that the original backbone is present
 +
*** So it was not properly digested
 +
** No growth was visible on the CM plate with the ligated I1352 with the CM resistant backbone pSB4C5
 +
*** If the digestion did not work properly, then the backbone could not be ligated with the I1352's promoter and RET
 +
** Conclusion:
 +
*** The enzymes are bad
 +
*** A new kit is being ordered
 +
*** Plans need to be made to repeat the Digestion and Ligation process with all promoters when kit arrives
 +
[[User:Trentay|Trentay]] 15:38, 31 July 2009 (UTC)
----
----
 +
 +
 +
===Tasks===
 +
''Afton and Maria''
 +
*
 +
----
 +
====Wetlab====
 +
''Afton and Maria''
 +
*

Revision as of 15:38, 31 July 2009

Contents

Tuesday 7 July 2009

Results

Maria and Afton

  • Overnight culture of I1352 grew, but no visible florescence
  • Transformation Results below
Name Plate Purpose Growth Observation
NEB 10 beta LB (+) Control shocked lawn
NEB 10 beta LB (+) Control lawn
NEB 10 beta AMP (-) Control no growth
NEB 10 beta CM (-) Control no growth
NEB 10 beta CM I1352 ligated to the backbone pSB4C5 no growth
NEB 10 beta AMP I1352 ligated to the backbone pSB4C5 hundreds of colonies; some expressing RFP
NEB 10 beta AMP I1352 ligated with itself hundreds of colonies; some expressing RFP

Trentay 15:25, 31 July 2009 (UTC)

  • Interpretation of Transformation Results:
    • Shows the digestion/ligation process is not working
    • Because the I1352 grew successfully on an AMP plate after being 'ligated' with pSB4C5, shows that the original backbone is present
      • So it was not properly digested
    • No growth was visible on the CM plate with the ligated I1352 with the CM resistant backbone pSB4C5
      • If the digestion did not work properly, then the backbone could not be ligated with the I1352's promoter and RET
    • Conclusion:
      • The enzymes are bad
      • A new kit is being ordered
      • Plans need to be made to repeat the Digestion and Ligation process with all promoters when kit arrives

Trentay 15:38, 31 July 2009 (UTC)


Tasks

Afton and Maria

Wetlab

Afton and Maria

Retrieved from "http://2009.igem.org/Virginia_Commonwealth/31_July_2009"