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Team:DTU Denmark
From 2009.igem.org
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<h3>The Redoxilator</h3> | <h3>The Redoxilator</h3> | ||
| - | The aim of this project is to model and construct a molecular NAD+/NADH ratio sensing system in Saccharomyces cerevisiae by a synthetic biology approach. The sensor will work as an inducible transcription factor, being active only at certain levels of the NAD+/NADH ratios. By coupling the sensor to the expression of a yeast optimized fast degradable GFP, the system can be used for monitoring the NAD+/NADH redox poise in vivo. Other applications of the system could be heterologous redox coupled protein production in yeast. | + | The aim of this project is to model and construct a molecular NAD<sup>+</sup>/NADH ratio sensing system in Saccharomyces cerevisiae by a synthetic biology approach. The sensor will work as an inducible transcription factor, being active only at certain levels of the NAD<sup>+</sup>/NADH ratios. By coupling the sensor to the expression of a yeast optimized fast degradable GFP, the system can be used for monitoring the NAD<sup>+</sup>/NADH redox poise in vivo. Other applications of the system could be heterologous redox coupled protein production in yeast. |
<h3>The USER fusion standard</h3> | <h3>The USER fusion standard</h3> | ||
| - | Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on | + | Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER<sup>TM</sup> cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any ‘scars’ from the restriction enzyme digestions. Furthermore we will provide a software for easing the process of designing primers for the purpose of USER fusion. |
</html> | </html> | ||
Revision as of 13:47, 3 August 2009
