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LOVTAP
From 2009.igem.org
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| - | Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence <font size=3><span style="color: | + | Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence <font size=3><span style="color:orange"> Mél </span></font size> |
<br> Design a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. Design a switch on/off read out. | <br> Design a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. Design a switch on/off read out. | ||
Revision as of 09:25, 27 May 2009
To find
- Sequence of the 12 mutant fusion proteins or LOV domain AND TrpR (separately) => PUBMED (http://www.ncbi.nlm.nih.gov/)
To do
- - Find the exact genetic circuit for Trp repressor Nath
- - Write an e-mail to Strickland et al to ask Basile first shot; I think we could then look at it all together
Sequence (of all 12 fusion proteins)
Purification protocol
Digest assay protocol
- - Biobricks
Look for a (or many) paper(s) that characterizes E.Coli Trp repressor, and find the Trp operon sequence Mél
Design a biobrick that coexpresses LOVTAP and mCherry (after Trp operon) when LOVTAP bind the Trp operon. Design a switch on/off read out.
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