From 2009.igem.org
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| - | Pqrr4+I13500(RBS+GFP) was constructed to verify the Mutants; however, we realized that both of our parts were in high copy plasmids, which a single cell can take one of. Thus the smaller Pqrr4+I13500 circuit is chosen to be plasmid switched into another vector (pSB2K3), which was obtained from the Q04510 (inverter). Today, both the Pqrr4+13500 and pSB2K3 were cut at xbaI+PstI via restriction digest, and it is going to be left in the waterbath(37˚C) overnight. | + | <i>Pqrr4</i>+I13500(RBS+GFP) was constructed to verify the Mutants; however, we realized that both of our parts were in high copy plasmids, which a single cell can take one of. Thus the smaller <i>Pqrr4</i>+I13500 circuit is chosen to be plasmid switched into another vector (pSB2K3), which was obtained from the Q04510 (inverter). Today, both the Pqrr4+13500 and pSB2K3 were cut at xbaI+PstI via restriction digest, and it is going to be left in the waterbath(37˚C) overnight. |
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| - | Preparation for Plasmid switch of Pqrr4+I13500
| + | Overnight cultures of our reporter circuit |
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| - | | + | In order to further verify our reporter circuit (<i>Pqrr4</i>+B0034+K082003 Colony 9) using restriction digest, an overnight culture needs to be grown; thus 2 were grown and are to be shaken overnight at 37˚C |
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| | + | Modelling meeting |
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| | + | We had our first modelling dedicated meeting today, collaberating with the membrane computing team. We becan clearly outlining the differences and advantages/disadvantages of membrane computing and our methods of modelling. |
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Revision as of 22:31, 4 August 2009
University of Calgary
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CAROL
Modelling Meeting
- No lab experiments performed today (have to re-consider methods) - no colonies were found from the transformation that occured yesterday morning.
- For the modelling meeting, the following points were discussed:
1. A better organization of our work is required. We have both characterization and simulation from the mathematical team, but the work seems unlinked and not organized. Will have a more organized report for next week.
2. Greater detail was explained to us by Thane Kubik regarding the biochemical details that are behind the signalling pathway.
3. We need to start thinking about how we are going to present our two modelling system that we have created.
4. We will meet again on thursday and Afshin will give us a better overview of membrane computing.
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CHINMOYEE
Modellin is awesome
This is what you did
If you write bold, you will get bold text.
(what I did gives you spaces)
And this gives you italic.
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EMILY
Descriptive Title of What You're Doing
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FAHD
Marketing for August 4th
Today, I put my energy towards marketing our project. I made preparations for our 2009 iGEM fundraising Bake Sale which will be held tomorrow (Wednesday August 5th 2009).
I also worked on some grant stream applications such as t=The Enbridge Pipeline Inc. Community Support Grants and also contacted some Oil & Gas companies. The following is the list of companies I contacted today:
1)Mackenzie Aborginal Corporation (MAC)
2) KMC Mining
3) Imperial Oil Resources.
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
Preparation for Plasmid switch of Pqrr4+I13500
Pqrr4+I13500(RBS+GFP) was constructed to verify the Mutants; however, we realized that both of our parts were in high copy plasmids, which a single cell can take one of. Thus the smaller Pqrr4+I13500 circuit is chosen to be plasmid switched into another vector (pSB2K3), which was obtained from the Q04510 (inverter). Today, both the Pqrr4+13500 and pSB2K3 were cut at xbaI+PstI via restriction digest, and it is going to be left in the waterbath(37˚C) overnight.
Overnight cultures of our reporter circuit
In order to further verify our reporter circuit (Pqrr4+B0034+K082003 Colony 9) using restriction digest, an overnight culture needs to be grown; thus 2 were grown and are to be shaken overnight at 37˚C
We had our first modelling dedicated meeting today, collaberating with the membrane computing team. We becan clearly outlining the differences and advantages/disadvantages of membrane computing and our methods of modelling.
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MANDY
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PATRICK
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PRIMA
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STEFAN
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VICKI
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WIKI CODING HERE
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