Team:Paris/Freezer PCR products
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(New page: {{Template:Paris2009}} {{Template:Paris2009_menu}} ==Freezer== <center> Primers - PCR products - [[Team:Paris/Fr...) |
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|width=10%| [O21] Ompa_linker_F | |width=10%| [O21] Ompa_linker_F | ||
|width=10%| [O22] Ompa_linker_R | |width=10%| [O22] Ompa_linker_R | ||
- | |width=5%| | + | |width=5%| 80°C |
|width=10%| Matrix | |width=10%| Matrix | ||
- | |width=10%| | + | |width=10%| 525bp |
|width=25%| For standardisation of OmpA linker (BBaK103006) | |width=25%| For standardisation of OmpA linker (BBaK103006) | ||
|width=5%| ? | |width=5%| ? |
Revision as of 23:28, 4 August 2009
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)
Color code for sticker | |||||||
---|---|---|---|---|---|---|---|
Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
PCR products
PCR products | ||||||||||
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Number | Name | Oligo F | Oligo R | Tm | Matrix | Length | Comments | Quantity | []ng/µL | Date |
A1 | g3p_std | [O19] g3p_F | [O20] g3p_R | Tm | Matrix | Length | For standardisation of g3p and adding SacI site | ? | ? ng/µL | ? |
A2 | OmpA_L_std | [O21] Ompa_linker_F | [O22] Ompa_linker_R | 80°C | Matrix | 525bp | For standardisation of OmpA linker (BBaK103006) | ? | ? ng/µL | ? |
http://parts.mit.edu/r/parts/images/partbypart/icon_terminator.png
http://parts.mit.edu/images/partbypart/icon_regulatory.png
http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png
http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png
http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
http://parts.mit.edu/registry/images/2/2e/Part_icon_generator.png