Revision as of 08:01, 6 August 2009

Home | Background | Project | Parts | Team

Diary | Protocols | Downloads | Brainstorm

Lé Protocols

Primer PCR protocol - We used it for: Making a full RIP molecule from our oligos

Purification protocol from gel - We used it for: Gel purification, over and over and over....

Making cells competent - We used it for: Making competent cells for both transformation and electroporation.

Transformation - We used it for: Transforming plasmids (containing biobricks) into competent cells.

Electroporation - We used it for: Transforming plasmids (containing biobricks) into competent cells. Electroporation is our preferred technique, since it requires less plasmid volume and uses electricity!

Miniprep - We used it for: Purifying plasmids from inoculations of transformed bacteria.

How to make an agarose gel - We used it for: Making Agarose gels, for use in testing brick and plasmid sizes. Very central technique to controlling your lab work.

Brainstorm:

Forslag til opbygning:

@@ Line 15: / Line 15: @@
 [[Team:SDU-Denmark/Protocols/Transformation|Transformation]] - We used it for: Transforming plasmids (containing biobricks) into competent cells.
+[[Team:SDU-Denmark/Protocols/Electroporation|Electroporation]] - We used it for: Transforming plasmids (containing biobricks) into competent cells. Electroporation is our preferred technique, since it requires less plasmid volume and uses electricity!
+[[Team:SDU-Denmark/Protocols/Miniprep|Miniprep]] - We used it for: Purifying plasmids from inoculations of transformed bacteria.
+[[Team:SDU-Denmark/Protocols/Agarose_Gel|How to make an agarose gel]] - We used it for: Making Agarose gels, for use in testing brick and plasmid sizes. Very central technique to controlling your lab work.
 '''Brainstorm:'''