Team:KULeuven/Freezer
From 2009.igem.org
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|{{kulpart|BBa_I742109}}||pSB1A2||COMT + rbs||plasmid from registry||||||August 6 | |{{kulpart|BBa_I742109}}||pSB1A2||COMT + rbs||plasmid from registry||||||August 6 | ||
|- | |- | ||
- | |||pSB1A2||ech + rbs||purified digest||||||August 6 | + | |||pSB1A2||ech + rbs||purified digest||||16,9 ng/µl||August 6 |
|- | |- | ||
- | |||||fcs + rbs||purified digest||||||August 6 | + | |||||fcs + rbs||purified digest||||16,6 ng/µl||August 6 |
|} | |} | ||
Revision as of 13:27, 6 August 2009
Contents |
Blue light receptor
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
MC4100 | / | strain of E.coli | july 24 | |||
2171 | / | forward primer for | 90µl | 20μM | july 24 | |
2172 | / | reverse primer for | 90µl | 20μM | july 24 | |
2171 | / | forward primer for | 100μM | july 24 | ||
2172 | / | reverse primer for | 100μM | july 24 | ||
pSB1A2 | GFP + rbs + terminator | purified | july 29 | |||
pSB1A2 | GFP + rbs + terminator | restricted with EcoRI and XbaI | july 29 | |||
/ | BLR promoter region | restricted with EcoRI | july 29 | |||
/ | BLR promoter region | 4 microcentrifuge tubes | july 30 |
Key/antikey/lock
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
/ | GFP with LVA tag | purified | july 28 | |||
/ | tetR promoter | purified | july 28 |
vanillin synthesis
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
pSB1A2 | ech + rbs | purified plasmid | 108,8 ng/µl | August 4 | ||
pSB1A2 | fcs + rbs | purified plasmid | 113,9 ng/µl | August 4 | ||
pSB1A2 | Sam5 + rbs | received from Edinburgh | August 3 | |||
pSB1A2 | Sam5 + rbs, PstI site removed | purified plasmid | 101,3 ng/µl | August 5 | ||
pSB1A2 | Sam8 + rbs | purified plasmid | 95,7 ng/µl | August 5 | ||
pSB1A2 | Sam8 | purified plasmid | 81,5 ng/µl | August 5 | ||
pSB1A2 | COMT | purified plasmid | 122,9 ng/µl | August 5 | ||
pSB1A2 | COMT + rbs | plasmid from registry | August 6 | |||
pSB1A2 | ech + rbs | purified digest | 16,9 ng/µl | August 6 | ||
fcs + rbs | purified digest | 16,6 ng/µl | August 6 |
vanillin receptor
part | plasmid | description | status | μl left | concentration | date |
---|
miscellaneous
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
dNTP mix | ATP, GTP, TTP, CTP | 94 | 10μM | July 24 | ||
pSB1A2 | medium constitutieve promoter | July 23 | ||||
ampicilline |
Categories: Team:KULeuven/BioBricks | Team:KULeuven/BioBricks/BBa K238000 | Team:KULeuven/BioBricks/BBa E0240 | Team:KULeuven/BioBricks/BBa K145015 | Team:KULeuven/BioBricks/BBa K145201 | Team:KULeuven/BioBricks/BBa I742113 | Team:KULeuven/BioBricks/BBa I742115 | Team:KULeuven/BioBricks/BBa I742105 | Team:KULeuven/BioBricks/BBa K238007 | Team:KULeuven/BioBricks/BBa I742106 | Team:KULeuven/BioBricks/BBa I742142 | Team:KULeuven/BioBricks/BBa I742107 | Team:KULeuven/BioBricks/BBa I742109 | Team:KULeuven/BioBricks/J23110