Template:Team:KULeuven/6 August 2009/VanillinProduction

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(New page: Today the purification of the gel electrophorese was done on the cells from yesterday, with the nanodrop the concentration was measured:<br> A1: 11,6 ng/ul <br> Sam5 A2: 5,5 ng/ul<br>Sam8 ...)
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Revision as of 14:26, 6 August 2009

Today the purification of the gel electrophorese was done on the cells from yesterday, with the nanodrop the concentration was measured:
A1: 11,6 ng/ul
Sam5 A2: 5,5 ng/ul
Sam8 B2: 24,3 ng/ul
ComT C1: 16,6 ng/ul
Fsc C2: 16,9 ng/ul
Ech

A1 and A2 showed a very low concentration and the 260/280 value was quite high so both were electrophoresed again. B1 (RBS binding site) had failed because it was too short, however ligating B1 and B2 (ComT) was no longer needed since the part with RBS was available in the iGEM 2009 kit, .