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Template:Team:KULeuven/6 August 2009/VanillinProduction
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A1 and A2 showed a very low concentration and the 260/280 value was quite high so both were electrophoresed again. | A1 and A2 showed a very low concentration and the 260/280 value was quite high so both were electrophoresed again. | ||
B1 (RBS) had failed because it was too short, however ligating B1 and B2 (ComT) was no longer needed since the part with RBS was available in the iGEM 2009 kit, {{kulpart|BBa_I742109}}. | B1 (RBS) had failed because it was too short, however ligating B1 and B2 (ComT) was no longer needed since the part with RBS was available in the iGEM 2009 kit, {{kulpart|BBa_I742109}}. | ||
| + | <br> | ||
| + | A1 and A2 were redigested:<br> | ||
| + | A1: '''Sam8''', EcoRI en SpeI<br> | ||
| + | A2: '''Sam5''', EcoRI en XbaI<br> | ||
| + | followed by gel electrophorese | ||
Revision as of 14:30, 6 August 2009
Today the purification of the gel electrophorese was done on the cells from yesterday, with the nanodrop the concentration was measured:
A1, Sam5: 11,6 ng/ul
A2, Sam8: 5,5 ng/ul
B2, ComT: 24,3 ng/ul
C1, Fsc: 16,6 ng/ul
C2, Ech: 16,9 ng/ul
A1 and A2 showed a very low concentration and the 260/280 value was quite high so both were electrophoresed again.
B1 (RBS) had failed because it was too short, however ligating B1 and B2 (ComT) was no longer needed since the part with RBS was available in the iGEM 2009 kit, .
A1 and A2 were redigested:
A1: Sam8, EcoRI en SpeI
A2: Sam5, EcoRI en XbaI
followed by gel electrophorese
