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TAE Buffer
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(New page: {{UIUC09Temp}} {{Illinois}} {{IllExpNav}} [https://2009.igem.org/Team:Illinois/Protocols#Recipes Back to Protocols page] <html> <div id="uicontentbox"> </html> ==1x TAE Buffer== *242g Tri...)
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(New page: {{UIUC09Temp}} {{Illinois}} {{IllExpNav}} [https://2009.igem.org/Team:Illinois/Protocols#Recipes Back to Protocols page] <html> <div id="uicontentbox"> </html> ==1x TAE Buffer== *242g Tri...)
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Revision as of 18:04, 6 August 2009
1x TAE Buffer
- 242g Tris base
- 57.1mL glacial acetic acid
- 100mL 0.5M EDTA (pH 8.0)
- 1L dH2O
Dissolve Tris base in 750mL of deionized water. Add the acetic acid and EDTA, and adjust the volume to 1L by adding water. The final pH should be about 8.5.
