August/6 August 2009

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(→Design genetic circuits)
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==Design genetic circuits==
==Design genetic circuits==
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We take notice of signal molecules (3OC6HSL,N-butylyl-HSL,3OH,C14:1-HSL,Al-1).So we
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We take notice of signal molecules. So I put them in order in the chart below.
 +
 
 +
　Indcer  signal moleculer  Receiver
 +
　　LuxI ⇒　    3OC6HSL　    　⇒ LuxR
 +
  LasI ⇒　　AI-1(3OC12HSL) 　⇒　LasR
 +
  CinI  ⇒  　3OH,C14:1-HSl  　⇒　CinR

Revision as of 21:58, 6 August 2009

Contents

Morning Meeting

To do in lab

1. Transform & Selection

  • 培地作り
    • LB
    • LB Amp+
    • LB Kan+
  • Transformation
    • About 16 kinds of parts

2. Breeding

3. Refinement

To bring

  • Timer
  • Pen
  • Slipper

Design genetic circuits

We take notice of signal molecules. So I put them in order in the chart below. 

　Indcer   signal moleculer   Receiver

　　LuxI ⇒　 3OC6HSL　 　⇒ LuxR 

  LasI ⇒　　AI-1(3OC12HSL) 　⇒　LasR
 CinI  ⇒  　3OH,C14:1-HSl  　⇒　CinR
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