August/6 August 2009

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(Difference between revisions)
(→Design genetic circuits)
(→Design genetic circuits)
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We take notice of signal molecules. So I put them in order in the chart below.<br/>
We take notice of signal molecules. So I put them in order in the chart below.<br/>
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　                                                                                                                                Indcer   signal moleculer  Receiver  
+
　                                                                                                                                Inducer   signal moleculer  Receiver  
  * LuxI ⇒　    3OC6HSL　    　⇒ LuxR
  * LuxI ⇒　    3OC6HSL　    　⇒ LuxR
  *  LasI ⇒　　AI-1(3OC12HSL) 　⇒　LasR
  *  LasI ⇒　　AI-1(3OC12HSL) 　⇒　LasR
  * CinI  ⇒  　3OH,C14:1-HSl  　⇒　CinR
  * CinI  ⇒  　3OH,C14:1-HSl  　⇒　CinR
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  * RhlI ⇒　AI-1(C4HSL)   ⇒　RhlR
+
  * RhlI ⇒　　AI-1(C4HSL) 　　 ⇒　RhlR  
 +
* （例外）
 +
　　　

Revision as of 22:12, 6 August 2009

Contents

Morning Meeting

To do in lab

1. Transform & Selection

  • 培地作り
    • LB
    • LB Amp+
    • LB Kan+
  • Transformation
    • About 16 kinds of parts

2. Breeding

3. Refinement

To bring

  • Timer
  • Pen
  • Slipper

Design genetic circuits

We take notice of signal molecules. So I put them in order in the chart below.
　 Inducer signal moleculer Receiver 

* LuxI ⇒　    3OC6HSL　     　⇒ LuxR
*  LasI ⇒　　AI-1(3OC12HSL) 　⇒　LasR
* CinI  ⇒  　3OH,C14:1-HSl  　⇒　CinR
* RhlI ⇒　　AI-1(C4HSL)  　　 ⇒　RhlR 
* （例外）

　　　

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