August/6 August 2009

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(→To do in lab)
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===To do in lab===
===To do in lab===
1. Transform & Selection
1. Transform & Selection
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*培地作り
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*Medium buildng
**LB
**LB
**LB Amp+
**LB Amp+
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*Transformation
*Transformation
**About 16 kinds of parts
**About 16 kinds of parts
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     We transform thesefollowing parts today.
+
     We transform these following 14 parts today.
       B0015 Plate 1 23L    S03878 Plate2 16M
       B0015 Plate 1 23L    S03878 Plate2 16M
       C0179 Pate 2 8M    C0070 Plate2  12H
       C0179 Pate 2 8M    C0070 Plate2  12H

Revision as of 23:55, 6 August 2009

Contents

Morning Meeting

To do in lab

1. Transform & Selection

  • Medium buildng
    • LB
    • LB Amp+
    • LB Kan+
  • Transformation
    • About 16 kinds of parts 
   We transform these following 14 parts today.
      B0015 Plate 1 23L    S03878 Plate2 16M
      C0179 Pate 2 8M     C0070 Plate2  12H
     F1610 Plate2 24G    B0034 Plate1 2M
      I0462 Plate1 8O    C0078 Plate1 14D
     C0077 Plate1 14A   I1466 Plate1 23J

2. Breeding

3. Refinement

To bring

  • Timer
  • Pen
  • Slipper

Design genetic circuits

We take notice of signal molecules. So I put them in order in the chart below.
　 *Inducer ⇒（synthesize) signal moleculer ⇒（synthesize) Receiver 

* LuxI ⇒　    3OC6HSL　     　⇒ LuxR
*  LasI ⇒　　AI-1(3OC12HSL) 　⇒　LasR
* CinI  ⇒  　3OH,C14:1-HSl  　⇒　CinR
* RhlI ⇒　　AI-1(C4HSL)  　　 ⇒　RhlR 
* （exception）agrD synthesize AIP with agrB.AIp synthesize agrC . AgrA which is synthesized by agrC activate promoter.

 From now on,We have to do what I mention below
 1.Search other cell-cell comunication system
 2.Check systems component completely
 3.Create new systems or reinforce cell funcution with additional ideas
 4.Search the effect of cross talk 

          written by Tadasi Nakamura
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